Porous Substrate Capture for High-Throughput Single-Cell Multiplex Analysis
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Solution Overview
Problem
There is a lack of a practical, cost-effective, high-throughput technique to examine and detect bio-molecular components of individual cells or viruses, limiting biotechnology and biomedical research.
Innovation Solution
A method involving covalent capture and detection of bio-molecular components of single cells or viruses on a porous solid substrate, utilizing electrophoresis, convection, or centrifugation to transfer components into interstices or pores, followed by covalent binding and probing with specific probes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional techniques are used to examine bio-molecular components of individual cells, then measurement capability is achieved, but throughput is low and cost is high
Solution Approach 1:
The invention segments the examination process by capturing multiple individual cells simultaneously on a single substrate with multiple pores, allowing parallel processing of multiple cells rather than sequential examination, thereby increasing throughput while maintaining individual cell analysis capability
Solution Approach 2:
The substrate serves multiple functions: it captures cells, contains pores for component transfer, provides surface for covalent binding, and enables multiplexed detection. This multi-functionality reduces the need for multiple separate devices or processes, simplifying the overall system while maintaining high throughput
2Productivity
If individual cell analysis is performed with high throughput, then productivity increases, but cost effectiveness decreases
Solution Approach 1:
The invention creates multiple copies of the analysis capability on a single substrate, with each pore representing a replication of the cell capture and analysis function. This allows simultaneous processing of multiple cells using identical protocols and reagents, reducing per-sample cost while maintaining high throughput
Solution Approach 2:
The invention changes the scale parameter from single-cell sequential analysis to multi-cell parallel analysis on a single substrate. By increasing the number of pores and cells processed simultaneously, the system achieves economies of scale that improve cost effectiveness while maintaining individual cell resolution
3Measurement precision
If bio-molecular components are transferred to porous substrate for capture, then detection capability is improved, but sample processing complexity increases
Solution Approach 1:
The invention extracts the bio-molecular components from the complex cellular environment and transfers them to the simplified porous substrate environment. This separation allows detection to occur in a controlled setting with reduced background interference, improving measurement precision while the automated transfer process minimizes additional processing complexity
Solution Approach 2:
The porous substrate acts as an intermediary between the cell and the detection system. It facilitates the transfer and capture of bio-molecular components through its pore structure and covalent binding capabilities, enabling precise detection without requiring direct interaction between the detection reagents and the intact cell, thus simplifying the overall process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-throughput examination and detection of multiple bio-molecular components of individual cells or viruses, allowing for repeated probing and quantification, overcoming the limitations of existing techniques.
Implementation Method 1
transferring the bio-molecular components of the biological sample is accomplished through electrophoresis
Implementation Method 2
transferring the bio-molecular components of the biological sample is accomplished through convection
Implementation Method 3
transferring the bio-molecular components of the biological sample is accomplished through centrifugation
Implementation Method 4
affixing (e.g., via covalent binding) the bio-molecular components of the biological sample to the interstices or pores of the solid substrate
Data Source
AI summary
The present disclosure provides methods of transferring bio-molecular components of individual cells in a biological sample to a solid porous substate. The method including contacting the biological sample to a first side of the porous solid substrate having a plurality of interstices or pores extending contiguously from the first side to a second side, transferring and affixing the bio-molecular components of the biological sample to the interstices or pores of the solid substrate. The present disclosure further provides methods of examining or detecting one or more bio-molecular components of individual cells in a biological sample. The method includes transferring one or more bio-molecular components of individual cells in a biological sample to a solid porous substate, and detecting one or more of the bio-molecular components of the biological sample.