Postbiotic Extract Production via Isoelectric Precipitation
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Solution Overview
Problem
Current methods for producing postbiotic extracts from probiotic microorganisms have low extraction efficiency and require large amounts of solvents, limiting their practical application in improving gut health and inhibiting biofilm formation.
Innovation Solution
A process involving the use of materials with specific isoelectric points to form a precipitate with a probiotic microorganism, followed by cell wall extraction treatment, to efficiently produce a postbiotic extract that can be used to improve gut health and inhibit biofilm formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional cell disruption methods (heat, enzymatic treatment, solvent extraction, sonication) are used to obtain postbiotics, then postbiotic extraction can be achieved, but the extraction efficiency is low and large amounts of solvents are required
Solution Approach 1:
The patent changes the pH parameter of the extraction medium to be above the isoelectric point of the probiotic microorganism, which alters the charge state of cellular components and enables efficient postbiotic extraction without requiring harsh solvents or extreme conditions, thereby improving extraction efficiency while reducing solvent consumption
Solution Approach 2:
The patent uses a pH-adjusted aqueous medium as an intermediary substance to facilitate the extraction of postbiotics from probiotic microorganisms, replacing traditional organic solvents and enabling efficient separation through pH-dependent solubility changes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process significantly increases the yield and protein content of postbiotic extracts, effectively improving gut health and inhibiting biofilm formation, as demonstrated by higher extraction yields and protein contents compared to traditional methods.
Implementation Method 1
adding the second material into the mixture, followed by adjusting a pH value of the second material-added mixture to between the first isoelectric point and the second isoelectric point, so that a precipitate is formed
Data Source
AI summary
Disclosed herein is a process for producing a postbiotic extract, which includes providing a first material having a first isoelectric point ranging from pH 1 to pH 6 and a second material having a second isoelectric point ranging from pH 4 to pH 8, admixing the first material and a probiotic microorganism with water having a pH greater than the second isoelectric point, so as to form a mixture, adding the second material into the mixture and then adjusting a pH of the second material-added mixture to between the first and second isoelectric points so that a precipitate is formed, and subjecting the precipitate to a cell wall isolation treatment to obtain the postbiotic extract. Use of the postbiotic extract is also disclosed.