Postbiotic Composition via Multi-Fermentation for Stable Bioactivity
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Solution Overview
Problem
Existing probiotic products have poor heat tolerance and stability, leading to reduced shelf-life and safety concerns, particularly for vulnerable populations, and there is a need for postbiotic compositions with enhanced bioactive properties and improved stability.
Innovation Solution
A method involving multiple fermentations with different microorganisms, followed by inactivation and mixing of fermentation products to create a postbiotic composition, utilizing various microorganisms such as Lactobacillus and Bifidobacterium species, and incorporating additional ingredients like L-tryptophan and specific culture media.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If probiotic products use living microorganisms to confer health benefits, then biological activity is improved, but heat tolerance and stability deteriorate
Solution Approach 1:
The patent applies parameter changes by transitioning the microbial state from living to inactivated form. This fundamental parameter change allows the product to maintain biological activity through inactivated microbial components (cell walls, proteins, metabolites) while simultaneously achieving superior heat tolerance and stability, as the inactivated form is no longer susceptible to thermal degradation that affects living organisms.
Solution Approach 2:
The patent employs the principle of using inactivated microbial components rather than maintaining living microorganisms throughout the product shelf life. The inactivated microbial cells and their components serve as the active ingredients, providing the health benefits without requiring the microorganisms to remain alive, thus eliminating the stability issues associated with living probiotics.
2Reliability
If probiotic products use living microorganisms, then health benefits are provided, but shelf-life is reduced
Solution Approach 1:
The patent fundamentally changes the state parameter of the microorganisms from living to inactivated. This parameter change enables the product to provide health benefits through inactivated microbial components while achieving extended shelf-life, as the inactivated form does not require special conditions to maintain viability and is stable under normal storage conditions.
3Reliability
If probiotic products use living microorganisms, then biological functionality is achieved, but safety concerns increase for vulnerable populations
Solution Approach 1:
The patent uses inactivated microbial components instead of living microorganisms to deliver biological functionality. The inactivated cells and their components (cell walls, proteins, metabolites) provide the desired health benefits without posing safety risks to vulnerable or immunocompromised populations, as there is no risk of infection or unwanted microbial activity.
4Reliability
If multiple fermenting microorganisms are used in separate fermentations, then bioactive properties are enhanced, but manufacturing complexity increases
Solution Approach 1:
The patent applies segmentation by conducting separate fermentation processes for different microorganisms, with each fermentation producing specific inactivated microbial components. This segmentation allows for optimized production of each microbial type's unique bioactive compounds, which are then combined to create a composition with enhanced overall bioactive properties.
Solution Approach 2:
The patent combines multiple inactivated fermentation products from different microorganisms into a single postbiotic composition. This merging integrates the bioactive components from various microbial sources, creating a synergistic effect that enhances the overall therapeutic potential and breadth of biological activities in the final product.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a postbiotic composition with enhanced bioactivity, increased stability, and prolonged shelf-life, offering benefits for health promotion and therapeutic applications, including improved gut barrier function and immune response.
Implementation Method 1
fermenting the inoculated plurality of culture media under conditions suitable for fermentation, thereby obtaining a plurality of fermentation products each containing a different fermenting microorganism
Implementation Method 2
inactivating the fermenting microorganisms in the plurality of fermentation products to obtain a plurality of inactivated fermentation products
Data Source
AI summary
The disclosure provides methods of preparing a postbiotic composition comprising multiple (i.e., two or more) separate microbial fermentations, which may be performed in parallel or sequential steps.