Potato StCDF1.4 Co-Dominant Markers for Single-Primer Genotyping

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current molecular markers for identifying the StCDF1.4 genotype in potatoes are inefficient, requiring multiple primer pairs and labor-intensive analyses, and there is a lack of effective markers for distinguishing StCDF1.4 from other alleles, limiting potato breeding options for adaptability to changing climatic conditions.

Innovation Solution

Development of co-dominant molecular markers using a single pair of primers (CDF1.4-prom F/R) that target a 623 bp deletion in the StCDF1.4 promoter, enabling rapid and accurate identification of homozygous or heterozygous StCDF1.4 genotypes through agarose gel electrophoresis, and introduction of CAPS markers for StCDF1.6 to enhance breeding efficiency.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If multiple primer pairs are used to identify StCDF1.4 genotype, then identification accuracy is improved, but device complexity and labor intensity increase

Engineering Contradiction:

Improveidentification accuracyVSAvoidmarker system complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent combines multiple primer pairs into a single integrated marker system (CDF1.4-prom F/R) that simultaneously detects the 623 bp deletion and the 7 bp insertion in StCDF1.4, reducing the number of separate assays while maintaining identification accuracy

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The single primer pair CDF1.4-prom F/R serves multiple functions: it amplifies the promoter region containing the deletion, detects the presence of StCDF1.4 allele, and can distinguish between homozygous and heterozygous genotypes, replacing what previously required multiple specialized primer pairs

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Reliability

If multiple primer pairs and tedious analyses are used, then identification reliability is improved, but loss of time and labor increase

Engineering Contradiction:

Improveidentification reliabilityVSAvoidanalysis time

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The marker system performs preliminary detection of the 623 bp deletion in the promoter region, which serves as a quick screen before more detailed analysis. The deletion is a stable, easily detectable feature that可以快速 identify StCDF1.4 carriers

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent replaces complex mechanical analysis procedures with a simplified PCR-based molecular marker system that uses standard laboratory techniques, eliminating the need for tedious manual analyses while maintaining reliability

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Measurement precision

If existing molecular markers are used, then identification capability is provided, but ease of operation deteriorates due to complex procedures

Engineering Contradiction:

Improvegenotype detection capabilityVSAvoidoperation simplicity

Core Design Contradiction:

Measurement precisionVSEase of operation

Solution Approach 1:

The patent segments the StCDF1.4 allele into two detectable features: the 623 bp promoter deletion and the 7 bp insertion. By focusing on the deletion in the promoter region, the system creates a clear, operable marker that is easier to detect than the insertion alone, simplifying the overall operation

Inventive Principle:

Principle #1Segmentation

4Productivity

If StCDF1.4 identification methods are improved, then breeding efficiency is enhanced, but manufacturing precision requirements increase

Engineering Contradiction:

Improvebreeding efficiencyVSAvoidmarker development precision

Core Design Contradiction:

ProductivityVSManufacturing precision

Solution Approach 1:

The patent changes the detection parameter from detecting the 7 bp insertion (which requires high precision and multiple primers) to detecting the 623 bp deletion in the promoter region. This parameter change allows for simpler, more robust detection that is easier to implement in breeding programs while maintaining efficiency

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The markers provide time-efficient, labor-saving, and environmentally independent identification of StCDF1.4 and StCDF1.6 genotypes, facilitating accurate breeding for long-day adaptation and reducing costs, while enabling non-destructive detection of potato seedlings and extensive screening in commercial breeding.

Implementation Method 1

The molecular markers may identify homozygous or heterozygous genotypes of StCDF1.4

Methodology Applied

Scientific EffectPCR amplification:

Implementation Method 2

The amplification products of the molecular markers provided in the present disclosure show significant differences in the size of characteristic bands. Whether the potato to be tested contains the StCDF1.4 gene and genotype thereof may be judged by agarose gel electrophoresis.

Methodology Applied

Scientific EffectAgarose gel electrophoresis: Electrophoresis

Data Source

PatentUS20260043093A1CO-DOMINANT MOLECULAR MARKERS FOR LONG-DAY ADAPTATION GENE StCDF1.4 IN POTATO AND USE THEREOF

Publication Date: 2026.02.12 AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI

AI summary

Co-dominant molecular markers for long-day adaptation gene StCDF1.4 in potato and the use thereof, which belongs to the technical field of molecular biology. The co-dominant molecular marker alone may identify homozygous or heterozygous genotypes of StCDF1.4, thus overcoming the limitation of existing molecular markers that require combinations of multiple pairs of primers to distinguish genotypes of StCDF1.4.