PRB1-Binding Antibody Sequences for Serum and Saliva Detection

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Solution Overview

Problem

Existing technologies are limited in their ability to detect human PRB1 protein in both saliva and serum effectively.

Innovation Solution

Development of antibodies and antigen-binding fragments with specific amino acid sequences capable of binding to human PRB1 protein, allowing for their detection in both saliva and serum using immunological methods such as ELISA and Western blotting.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing detection methods are used, then PRB1 protein can be detected in saliva, but detection in serum is not effective

Engineering Contradiction:

Improvedetection capabilityVSAvoidsample type range

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent modifies the amino acid sequences in the variable regions of the antibody (specifically the heavy chain variable region and light chain variable region) to change the binding characteristics. This parameter change enables the antibody to bind effectively to PRB1 protein in both saliva and serum, resolving the contradiction between detection reliability in saliva and adaptability to different sample types.

Inventive Principle:

Principle #35Parameter changes

2Measurement precision

If existing antibodies are used, then detection in saliva is achievable, but detection precision in serum is insufficient

Engineering Contradiction:

Improvedetection accuracyVSAvoidsample matrix compatibility

Core Design Contradiction:

Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The patent applies local quality by specifically modifying the variable regions (VD, VL, CDRs) of the antibody while keeping the constant regions unchanged. This localized modification approach allows the antibody to achieve high binding affinity and measurement precision for PRB1 in serum without losing its ability to detect PRB1 in saliva, thus resolving the contradiction between measurement precision and sample matrix compatibility.

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The antibodies enable accurate detection of human PRB1 protein in serum, providing a reliable marker for renal function assessment, particularly in chronic kidney disease diagnosis.

Implementation Method 1

an antibody or an antigen-binding fragment thereof having a capability of binding to a human PRB1 protein

Methodology Applied

Scientific EffectAntigen-antibody binding:

Data Source

PatentEP4678658A1Antibody or antigen-binding fragment of same

Publication Date: 2026.01.14 KARYDO THERAPEUTIX INC

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AI summary

An object of the present invention is to provide an antibody capable of detecting a human PRB 1 protein not only in saliva but also in serum. The problem is solved by the antibody or the antigen-binding fragment thereof having a capability of binding to the human PRB1 protein, wherein a heavy chain variable region includes an amino acid sequence that is 95% or more identical to a heavy chain variable region represented by an amino acid sequence from 19th to 132nd positions in SEQ ID NO: 1, and a light chain variable region includes an amino acid sequence that is 95% or more identical to a light chain variable region represented by an amino acid sequence from 21st to 132nd positions in SEQ ID NO: 6.