Preloaded Fluorescence Polarization Assay Support for Wide-Range Measurement
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Solution Overview
Problem
Existing fluorescence polarization immunoassays require complex operations due to the need for multiple reagents and have limited measurement ranges, necessitating sample dilution when concentrations fall outside the measurable range.
Innovation Solution
A support for fluorescence polarization immunoassay is developed with reaction parts pre-loaded with antibodies and fluorescent labeling substances, allowing simple operation by adding a sample solution, and featuring varied concentrations and binding affinities to expand the measurement range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple reagents are added to each well for fluorescence polarization immunoassay, then the assay can be performed with proper reagent composition, but the operation becomes complicated
Solution Approach 1:
The patent combines multiple reagents (antibody solution and fluorescent labeling substance solution) into a single integrated reagent that is pre-loaded in the reaction part. This merging eliminates the need for separate addition steps while maintaining the functional requirements of the assay, thus improving ease of operation without sacrificing assay accuracy
Solution Approach 2:
The reagents are prepared and loaded into the reaction part in advance before the actual assay is performed. The antibody and fluorescent labeling substance are pre-positioned in the reaction chamber, so when the sample is added, the reaction can proceed immediately without complex multi-step reagent additions during the measurement process
2Ease of manufacture
If a fixed concentration range is used for measurement, then the assay can be performed with standardized reagents, but sample dilution is required when concentrations fall outside the range
Solution Approach 1:
The patent employs multiple reaction parts with different reagent concentrations (e.g., first reaction part with concentration C1, second reaction part with concentration C2) to create a dynamic measurement system. This allows the assay to adapt to different sample concentrations without requiring pre-dilution, as the appropriate concentration range is already built into the system structure
Solution Approach 2:
The invention changes the concentration parameter of the reagents across different reaction parts. By having reaction parts with varying antibody or fluorescent labeling substance concentrations, the system can measure target substances across a broader concentration range without requiring sample dilution steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables simple and accurate measurement of target substance concentrations across a wider range without dilution, utilizing microfluidic channels and pH adjusters to enhance sensitivity and precision.
Implementation Method 1
Fluorescence polarization immunoassay is known as an immunity analysis method using fluorescence
Implementation Method 2
utilizes a significant change in the fluorescence polarization caused by a specific antigen-antibody reaction between this reagent and a fluorescently labeled antigen
Implementation Method 3
Fluorescence polarization measured by the fluorescence polarization immunoassay is known to be proportional to the effective volume of a target substance
Data Source
AI summary
Provided is a support for fluorescence polarization immunoassay of which reaction parts are loaded with an antibody and a fluorescent labeling substance. The plurality of reaction parts may be loaded with different concentrations of an antibody and a fluorescent labeling substance. Further, antibodies having different binding affinities for a target substance may be loaded. With such a support, fluorescence polarizations can be measured simply by adding a sample solution containing a target substance to the reaction parts, and a wide measurement range of the concentration the target substance can be secured.