Preparative Separation Liquid Chromatograph System
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Solution Overview
Problem
Determining preparative separation conditions for liquid chromatograph systems is cumbersome and requires expertise, especially for unskilled users, as it involves adjusting conditions based on complex chromatogram analysis and understanding of column and mobile phase properties.
Innovation Solution
A preparative separation liquid chromatograph system with a chromatographing section, chromatogram creating section, storage section, index input section, and preparative separation condition determining section that uses a database to store and analyze chromatogram data from standard samples to determine optimal preparative separation conditions for target samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional method of determining preparative separation conditions by repeating LC analysis and manual adjustment is used, then separation conditions can be determined, but the process becomes extremely cumbersome and time-consuming
Solution Approach 1:
The system performs preliminary LC analysis under various conditions using a search column to obtain chromatogram data before actual preparative separation. This preliminary data collection enables automatic condition determination without requiring manual trial-and-error analysis during the separation process, significantly reducing the time needed for condition determination while maintaining accuracy.
Solution Approach 2:
The invention creates a database that stores and copies chromatogram data from preliminary analyses. Instead of requiring users to manually perform and interpret multiple LC analyses, the system uses stored chromatogram data as copies to automatically determine optimal separation conditions, transforming a manual iterative process into an automated data retrieval and processing operation.
2Ease of operation
If manual adjustment of analysis conditions is performed by unskilled persons, then condition determination is possible, but the complexity of understanding column and mobile phase properties creates significant difficulty
Solution Approach 1:
The system introduces an intermediary layer between the user and the complex chromatogram analysis. The computer automatically processes chromatogram data, compares it with stored data, and determines optimal conditions without requiring users to understand column and mobile phase properties. This intermediary automation layer simplifies operation while managing the inherent complexity of the system.
Solution Approach 2:
The system performs self-service by automatically determining separation conditions based on stored chromatogram data without requiring user expertise. The computer independently analyzes data, compares it with database entries, and selects appropriate conditions, eliminating the need for users to manually interpret complex chromatograms or understand underlying chromatographic theory.
3Quantity of substance
If a column with smaller capacity is used for initial analysis, then sample amount can be reduced, but the adjusting process becomes more complex requiring repeated analysis
Solution Approach 1:
The system performs preliminary LC analysis using a search column with smaller capacity to obtain chromatogram data for various standard samples under different conditions. This preliminary action collects essential separation information without requiring large sample amounts, and the stored data enables automatic condition determination for preparative separation without subsequent manual adjustment.
Solution Approach 2:
The invention stores and copies chromatogram data from preliminary analyses in a database. These copied data serve as reference information for automatically determining optimal separation conditions, eliminating the need to repeat analysis adjustments. The copied chromatogram data enables direct determination of preparative conditions without iterative manual optimization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables users without extensive knowledge to easily set preparative separation conditions by performing liquid chromatograph analysis in a searching mode and referencing stored data, simplifying the process of separating and collecting components.
Implementation Method 1
a liquid chromatograph (which is hereinafter abbreviated as 'LC') in which a fraction collector is provided on the downward side of the LC passage so as to separate one or more target components contained in a sample
Implementation Method 2
The separated target components are detected with a detector (e.g. spectrophotometer) provided on the downward side of the column
Data Source
AI summary
Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.