Primary Amine Compounds Neutralize Carbapenems in Culture Media
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Solution Overview
Problem
Current methods for culturing and detecting microorganisms are hindered by the presence of antibiotics, such as β-lactams, which interfere with the recovery and identification of microorganisms, particularly in cases of sepsis, as existing resins and non-resinous adsorbents are ineffective in neutralizing carbapenems.
Innovation Solution
The use of primary amine-containing compounds, including non-thiol containing amines, is introduced to neutralize, bind, or inhibit antibiotics like carbapenems in culture media, allowing for the effective detection of microorganisms by adding these compounds to the culture medium before inoculation with a test sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If resins and non-resinous adsorbents are used to remove antibiotics from blood samples, then the removal of inhibitors is improved, but carbapenems cannot be effectively neutralized
Solution Approach 1:
The patent changes the chemical parameter of the adsorbent material from traditional resins to primary amine-containing compounds. This parameter change enables the adsorbent to specifically bind and neutralize carbapenem antibiotics through chemical interaction between the primary amine groups and the beta-lactam ring structure of carbapenems, resolving the inability of conventional adsorbents to neutralize this specific antibiotic class.
Solution Approach 2:
The patent employs composite materials by combining primary amine-containing compounds with adsorbent matrices. This composite approach creates a material that possesses both the adsorption capabilities of traditional resins and the specific chemical reactivity of primary amines toward carbapenems, achieving both broad-spectrum inhibitor removal and selective carbapenem neutralization.
2Reliability
If antibiotics are administered to patients to treat infections, then the treatment of infections is improved, but the detection of microorganisms in cultured samples is hindered
Solution Approach 1:
The patent applies preliminary action by incorporating the primary amine-containing compound into the culture medium before inoculation with the patient sample. This pre-treatment ensures that any antibiotics present in the sample are neutralized immediately upon contact with the culture medium, creating an antibiotic-free environment that allows microorganisms to grow and be detected without interference from previously administered antibiotics.
Solution Approach 2:
The primary amine-containing compound acts as an intermediary substance between the antibiotic molecules and the microorganisms. It binds to the antibiotics through chemical interaction, forming a complex that is no longer biologically active, thereby protecting the microorganisms from antibiotic inhibition while allowing the antibiotics to remain present in the sample.
3Reliability
If culture media are autoclaved at very high temperatures to sterilize, then the sterilization is improved, but toxic by-products are formed that inhibit microorganism growth
Solution Approach 1:
The patent extracts or removes the harmful toxic by-products from the culture medium by incorporating primary amine-containing compounds that specifically bind to these by-products. This extraction process separates the beneficial sterilization effect from the harmful toxic effects, allowing the culture medium to maintain its sterilized state without the inhibitory toxic by-products that would otherwise prevent microorganism growth.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the neutralization of antibiotics, facilitating the growth and detection of microorganisms that would otherwise be suppressed, thereby enhancing the recovery and diagnosis of infections caused by microorganisms.
Implementation Method 1
The use of primary amine-containing compounds, including non-thiol containing amines, is introduced to neutralize, bind, or inhibit antibiotics like carbapenems in culture media
Data Source
AI summary
The present invention is directed to a method and means for the neutralization, binding, and/or inactivation of antimicrobials in a test sample. The invention is also directed to a method of detecting the presence of one or more microorganisms in a test sample by culturing the test sample in a culture media comprising one or more primary amine-containing compounds.