Probiotic Bacterial Composition for Newborn Animal Diarrhea Treatment
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Solution Overview
Problem
Newborn animals, particularly in farming, are prone to bacterial infections leading to diarrhea, which can be severe and life-threatening, and current treatments with antibiotics are not always effective, prompting a need for alternative methods to maintain animal health without relying heavily on antibiotics.
Innovation Solution
A composition comprising specific strains of lactic acid bacteria, such as CECT 8163, CECT 8165, CECT 8164, CECT 8166, CECT 8347, CECT 8348, CECT 8349, and CECT 8350, which exhibit antimicrobial activity, acid tolerance, and bile salts tolerance, administered to newborn mammals to treat or prevent diarrhea and promote weight gain, potentially used in combination with prebiotics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If antibiotics are used to treat bacterial infections in newborn animals, then the effectiveness of treatment is improved, but the harmful effects of antibiotic resistance and dysbiosis worsen
Solution Approach 1:
The patent uses probiotic bacteria (Lactobacillus reuteri, Lactobacillus plantarum, and Enterococcus faecium) as intermediary organisms to restore intestinal flora balance and compete with pathogenic bacteria. These probiotics act as mediators that indirectly combat bacterial infections by reinforcing the natural microbiome barrier, rather than directly killing pathogens with antibiotics. This approach treats the underlying dysbiosis condition while avoiding antibiotic resistance issues.
Solution Approach 2:
The patent converts the harmful effect of dysbiosis (caused by antibiotic use) into a beneficial treatment approach by using probiotic supplementation. The same antibiotic-induced disruption of intestinal flora that causes harm is addressed by introducing beneficial probiotic bacteria that can repopulate and balance the microbiome, turning the negative consequence into a therapeutic opportunity.
2Object-generated harmful factors
If antibiotics are reduced or eliminated in animal husbandry, then the harmful effects of antibiotic resistance are reduced, but the ability to treat severe bacterial infections worsens
Solution Approach 1:
The patent applies probiotic supplementation in advance (prophylactically) to newborn animals to establish a healthy intestinal microbiome before pathogenic infections can take hold. By pre-colonizing the gut with beneficial bacteria like Lactobacillus and Enterococcus species, the system creates natural biological defense mechanisms that prevent severe infections from developing, reducing the need for curative antibiotic treatments later.
3Object-generated harmful factors
If probiotics are used as an alternative to antibiotics, then the harmful effects are reduced, but the reliability of treatment for severe infections may worsen
Solution Approach 1:
The patent employs a composite probiotic formulation containing multiple bacterial species (Lactobacillus reuteri, Lactobacillus plantarum, and Enterococcus faecium) with different mechanisms of action. This composite approach combines the benefits of multiple probiotic strains that work synergistically - Lactobacillus species provide acid production and bacteriocin secretion, while Enterococcus faecium contributes additional antimicrobial peptides and competitive exclusion capabilities, creating a more reliable alternative treatment system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The bacterial composition effectively treats or prevents bacterial infections and promotes weight gain in newborn mammals, offering a viable alternative to antibiotic treatments, with demonstrated efficacy in reducing mortality and improving health outcomes without the use of antibiotics.
Implementation Method 1
shows an antimicrobial activity evidenced by at least one of the following inhibition zones: (i) 10 mm or more, for example 14 mm or more, for Salmonella, (ii) 9 mm or more, preferably 10 mm or more, for Listeria monocytogenes, (iii) 9 mm or more, preferably 10 mm or more, for Staphyloccocus aureus, (iv) 10 mm or more, for example 18 mm or more, for Escherichia coli
Implementation Method 2
is able to retain essentially the same viability during 3 hours of incubation at pH=3.5, or, alternatively at pH=2.5
Implementation Method 3
is able to retain essentially the same viability during 4 hours of incubation in presence of 0.45% bile extract, preferably at pH=8
Data Source
Figure 1~2
AI summary
The invention relates to products and compositions that may be beneficial in animal husbandry. Said products and compositions comprise microorganisms, such as bacteria, and probiotic bacteria in particular. Thus, provided herein are microbial strains, as well as selection criteria which will enable the skilled reader to find further strains useful in the present invention. The strains, as well as compositions comprising the same, may be administered to animals, farmed animals such as swine in particular. The administration may occur in the first days of life. By administration of the products or compositions of the inventions animal growth can be promoted and animal weight can be increased. Bacterial infections may also be prevented or treated by said compounds or compositions.