Probiotic Composition for EHP Infection Control in Shrimp
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Solution Overview
Problem
Current methods to prevent or treat Enterocytozoon hepatopenaei (EHP) infection in aquaculture animals, such as shrimps and crabs, are ineffective, and existing preventive strategies like improved feeding and water management do not satisfactorily reduce infection rates.
Innovation Solution
A composition containing Enterococcus faecium EF08, Lactobacillus acidophilus LA1063, and Lactiplantibacillus plantarum LP28, administered to aquaculture animals, effectively inhibits EHP growth by altering the intestinal microflora and enhancing immune response.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If preventive strategies such as improved feeding and water management are implemented, then the occurrence of EHP infection can be reduced, but the effectiveness is not satisfactory
Solution Approach 1:
The patent changes the biological parameters of the host by introducing specific probiotic strains (Enterococcus faecium EF08, Lactobacillus acidophilus LA1063, and Lactiplantibacillus plantarum LP28) that alter the intestinal microflora composition. This biological parameter change provides effective protection against EHP infection without requiring complex external preventive measures
Solution Approach 2:
The probiotic bacteria establish themselves in the shrimp's intestine and provide self-service protection by competing with EHP for resources and producing antimicrobial substances. The host animal's own microbiota becomes the defense mechanism, eliminating the need for complex external preventive strategies
2Reliability
If probiotics are used to alter intestinal microflora, then immune response is enhanced, but the specific efficacy against EHP infection was previously unknown
Solution Approach 1:
The patent employs a feedback mechanism where the probiotic bacteria modulate the host's immune system response based on the presence of EHP. The immune system is stimulated to produce specific responses that reduce EHP load in the hepatopancreas, creating a dynamic defense system that adapts to the infection level
Solution Approach 2:
The patent uses a composite probiotic formulation containing three different bacterial strains (Enterococcus faecium EF08, Lactobacillus acidophilus LA1063, and Lactiplantibacillus plantarum LP28) that work synergistically. Each strain contributes different mechanisms of action, creating a composite biological material with enhanced efficacy against EHP infection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition significantly reduces EHP load in shrimps, demonstrating efficacy in preventing and treating EHP infections.
Implementation Method 1
Probiotics, which may serve as a live microbial food ingredient, are beneficial to health, and can selectively stimulate the growth of native bacteria in an intestine of a subject, so as to change the composition, quantity or nature of intestinal microflora of the subject
Implementation Method 2
Probiotics, which may serve as a live microbial food ingredient, are beneficial to health, and can selectively stimulate the growth of native bacteria in an intestine of a subject, so as to change the composition, quantity or nature of intestinal microflora of the subject, thereby enhancing intestinal health and immune response thereof
Implementation Method 3
A composition containing Enterococcus faecium EF08, Lactobacillus acidophilus LA1063, and Lactiplantibacillus plantarum LP28, administered to aquaculture animals, effectively inhibits EHP growth by altering the intestinal microflora and enhancing immune response
Data Source
AI summary
A method against Enterocytozoon hepatopenaei infection includes administering to a subject in need thereof a composition containing Enterococcus faecium EF08 which is deposited at the China General Microbiological Culture Collection Center (CGMCC) under an accession number CGMCC 5549, Lactobacillus acidophilus LA1063 which is deposited at the CGMCC under an accession number CGMCC 18744, and Lactiplantibacillus plantarum LP28 which is deposited at the CGMCC under an accession number CGMCC 3346 in accordance with the Budapest Treaty.