Dry Probiotic Composition Stability via Hydrolyzed Protein Matrix
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Solution Overview
Problem
Current technologies face challenges in preserving the viability of probiotic bacteria during industrial manufacturing processes and long-term storage, especially under high temperature and humidity conditions, and there is a need for stable probiotic compositions suitable for special dietary uses that comply with regulatory standards.
Innovation Solution
A dry probiotic composition comprising viable probiotic microorganisms, hydrolyzed proteins, disaccharides, oligosaccharides, and polysaccharides, with a specific formulation that includes at least 40% hydrolyzed proteins, less than 30% disaccharides, 1-30% oligosaccharides, and 0.1-40% polysaccharides, which maintains viability of at least 1x10^10 CFU/g and has a viability loss of less than 1 log unit/g after 3 months at 40°C and 33% relative humidity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional encapsulation and stabilizing compounds are used to protect probiotic viability during manufacturing and storage, then probiotic stability is improved, but regulatory compliance for special dietary formulations is compromised
Solution Approach 1:
The patent removes prohibited substances (trehalose, alginate, milk proteins) from the formulation and replaces them with permitted alternatives (hydrolyzed plant proteins, allowed disaccharides, and specific polysaccharides) that are compliant with Codex Alimentarius regulations for infant formula, thereby resolving the contradiction between achieving probiotic stability and maintaining regulatory compliance
Solution Approach 2:
The patent creates a composite matrix system combining hydrolyzed plant proteins (40-80%), disaccharides (20-40%), oligosaccharides (5-30%), and polysaccharides (1-10%) that work synergistically to protect probiotics while using only ingredients permitted in special dietary formulations, thus achieving both stability and regulatory compliance
2Adaptability or versatility
If probiotics are stored at high temperature and humidity conditions, then distribution flexibility is improved, but probiotic viability is compromised
Solution Approach 1:
The patent incorporates a protective matrix of hydrolyzed plant proteins, disaccharides, oligosaccharides, and polysaccharides that acts as a cushioning system before exposure to harsh conditions, protecting probiotic cells from thermal and humidity stress during distribution and storage, thereby maintaining viability even under elevated temperature and humidity conditions
Solution Approach 2:
The patent modifies the physical and chemical parameters of the probiotic formulation by using a dry powder form with controlled water activity and a complex carbohydrate-protein matrix that changes the thermal and humidity resistance parameters of probiotic cells, enabling them to withstand high temperature and humidity during distribution while maintaining viability
3Stability of the object's composition
If intermediate moisture conditions are used in probiotic formulations, then product stability is improved, but probiotic protection under high temperature is compromised
Solution Approach 1:
The patent develops a composite system combining hydrolyzed plant proteins with multiple carbohydrate types (disaccharides, oligosaccharides, and polysaccharides) that creates a synergistic protective matrix capable of maintaining both intermediate moisture stability and high-temperature probiotic protection, overcoming the limitations of single-component systems
Solution Approach 2:
The patent creates different functional zones within the formulation where hydrolyzed plant proteins provide structural stability at intermediate moisture levels while the carbohydrate matrix (particularly disaccharides and oligosaccharides) provides localized protection to probiotic cells against thermal stress, achieving both product stability and probiotic protection simultaneously
Data Source
Figure 1
AI summary
A dry stable probiotic composition is provided. The composition comprises one or more viable probiotic microorganisms, one or more hydrolyzed proteins, one or more disaccharides, one or more oligosaccharides, and one or more polysaccharides, but not trehalose. The composition has viability of at least 1x1010 CFU/g, and a viability loss of less than 1 log unit/g after 3 months at a temperature of 40 °C and a relative humidity of 33%. Also provided are methods for preparing the dry stable probiotic composition.