Process Record Slide with Control Targets for Antibody Quantification
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Solution Overview
Problem
Existing process record slides for loose cells lack quality control measures to quantify primary antibody concentration and stability, leading to potential errors in immunohistochemical and cytochemical processes due to antibody degradation over time.
Innovation Solution
Incorporating control targets within sample wells on the slides, which can capture primary and secondary antibodies, and using an adhesive coating to ensure effective binding, allowing for the quantification of antibody concentration and stability assessment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If absorbance measurement at 280 nm is used to determine antibody concentration, then the concentration can be calculated, but the measurement cannot detect hinge breakdown or antibody degradation
Solution Approach 1:
The patent introduces control targets as intermediary elements that mediate between the antibody reagent and the measurement system. These control targets serve as known targets that capture antibodies, allowing the measurement system to assess both concentration and functionality separately, thereby resolving the contradiction between concentration measurement and functionality assessment
Solution Approach 2:
The control targets provide feedback information about antibody quality and functionality. By incorporating known targets with expected capture characteristics, the system receives feedback on whether antibodies are functioning properly, enabling detection of degradation that would not be visible through concentration measurement alone
2Measurement precision
If control targets are incorporated within sample wells, then quantitative measurement of primary and secondary stain reagents is enabled, but the slide structure becomes more complex
Solution Approach 1:
The patent merges the control function with the existing sample well structure by incorporating control targets directly within the sample wells. This integration allows control and sample areas to coexist in the same well space, enabling quantitative measurement without requiring separate control wells or additional structural components
Solution Approach 2:
The control targets serve multiple functions: they act as known targets for quantitative measurement of reagents, provide quality control references, and enable assessment of both primary and secondary antibody functionality. This multi-functionality reduces the need for separate control mechanisms
3Ease of operation
If antibody vials are kept at room temperature for overnight use, then convenience is improved, but antibody viability is significantly reduced
Solution Approach 1:
The control targets provide preliminary assessment capability, allowing users to evaluate antibody quality before full staining procedures. This enables early detection of degradation, preventing waste of time and resources on compromised antibodies while maintaining operational flexibility
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The control targets enable accurate determination of primary antibody concentration and stability, reducing errors in staining processes and extending the viability of antibodies by identifying degradation issues early.
Implementation Method 1
The slide incorporates an adhesive coating that covalently captures the cells when the cells settle down
Implementation Method 2
Incorporating control targets within sample wells on the slides, which can capture primary and secondary antibodies
Data Source
AI summary
The present application relates to process record slide and method of using the same for loose cells. A single to multi-region slide wherein each region contains one to four control targets and is suitable for processing through Special Stains, IHC or CC, and others. Each region may be bonded by a hydrophobic barrier to form a well. The slide has an adhesive coating suitable for capturing loose cells and cell debris.