Prodigiosin Treatment for Potato Virus Y Control
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Solution Overview
Problem
Current methods lack an effective solution for resisting potato virus Y (PVY), which causes significant economic losses by reducing crop yield and quality, and the anti-plant virus mechanism of prodigiosin is not well understood.
Innovation Solution
The novel use of prodigiosin, a secondary metabolite from Serratia marcescens, is applied as a medicine to prevent and control PVY by promoting the ubiquitination of the HSP70 protein, degrading its expression, and activating systemic resistance in plants, thereby inhibiting PVY infection and replication.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods are used to control PVY, then existing plant protection measures are applied, but they lack effectiveness against PVY infection
Solution Approach 1:
Prodigiosin acts as an intermediary substance that mediates between the plant host and PVY virus. It binds to the HSP70 protein, preventing the virus from utilizing this host factor for replication, thereby providing effective control without directly attacking the plant or virus in a harmful manner.
Solution Approach 2:
The invention changes the biochemical parameter of HSP70 protein through prodigiosin treatment, specifically modifying its ubiquitination status. This parameter change disables the virus's ability to exploit HSP70 for replication, achieving reliable PVY control while maintaining plant health and productivity.
2Reliability
If prodigiosin is applied to resist PVY, then systemic resistance is activated and HSP70 expression is degraded, but the anti-virus mechanism was previously unclear
Solution Approach 1:
The invention establishes a feedback mechanism where prodigiosin treatment leads to increased ubiquitination of HSP70 protein, which is then degraded by the proteasome system. This feedback loop provides clear mechanistic understanding: prodigiosin → ubiquitination of HSP70 → HSP70 degradation → inhibition of PVY replication → systemic resistance activation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The prodigiosin treatment significantly increases the ubiquitination level of the host plant, reducing HSP70 protein expression, activating natural immune activity, and inducing systemic resistance, effectively inhibiting PVY infection and replication, with a demonstrated resistance of up to 100% in treated plants.
Implementation Method 1
The prodigiosin treats plants infected with PVY, promotes the ubiquitination of the HSP70 protein of the host plant, degrades or inhibits the expression of the Hsp70 protein
Data Source
AI summary
The present invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel use of prodigiosin in resisting potato virus Y. Application of prodigiosin in preparing a medicine for preventing and controlling potato virus Y. The medicine for preventing and controlling the potyvirus is a liquid or solid preparation containing prodigiosin. It is clarified that PVY promotes its own replication and infection by recruiting host factor Hsp70 in the process of infecting the host, realizing virus infection and spread. Hsp70 plays an important role in the infection and replication of plant viruses. Based on the above results, it is further clarified that prodigiosin treatment can significantly increase the ubiquitination level of the plant host, promote the ubiquitination of HSP70 protein of the host, degrade or inhibit the expression of Hsp70 protein, activate the natural immune activity of the host plant.