Propofol Extraction Apparatus with Integrated Lysis and Sorbent Filtration
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for automating sample preparation in biological assays, such as propofol extraction from whole blood, are hindered by manual lysis and dilution steps that are prone to user error, biohazard risks, and inefficiencies in analyte recovery due to the need for precise metering and filtration, which restricts their use to specialized laboratories and limits their effectiveness in point-of-care settings.
Innovation Solution
An apparatus and method that combines osmotic and mechanical lysis within a fluidic system, using a sorbent material with affinity for the analyte to enhance extraction efficiency, allowing whole blood samples to be processed directly without pre-treatment, reducing the need for precise dilution and filtration, and incorporating a solid phase extraction cartridge for accurate analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual lysis and dilution steps are performed by skilled laboratory personnel, then analyte extraction accuracy is maintained, but the complexity of operation increases and limits use to specialized laboratories
Solution Approach 1:
The system performs automatic lysis and dilution through integrated fluidic pathways and pumping mechanisms, eliminating the need for manual intervention by skilled personnel while maintaining extraction accuracy through controlled automated processes
Solution Approach 2:
Manual mechanical lysis and dilution operations are replaced with automated fluidic systems including pumps, valves, and integrated mixing chambers that precisely control sample processing without requiring human manipulation
2Measurement precision
If precise metering and filtration are implemented in automated systems, then analyte recovery is improved, but the device complexity and cost increase
Solution Approach 1:
The system combines metering, lysis, and filtration functions into integrated fluidic modules where a single automated pathway performs multiple operations sequentially, reducing the number of separate components while maintaining analyte recovery through controlled processing
Solution Approach 2:
The fluidic system is designed with multi-functional components that perform metering, mixing, and filtration through integrated pathways, allowing a single apparatus to handle various sample preparation steps without requiring specialized equipment for each function
3Measurement precision
If manual sample preparation steps are required, then analyte extraction accuracy is maintained, but productivity and efficiency decrease
Solution Approach 1:
The automated system enables continuous sample processing through integrated fluidic pathways where samples are automatically transported, lysed, diluted, and filtered in sequence without interruption, eliminating the time losses associated with manual transfer and preparation steps while maintaining extraction accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach improves the accuracy and efficiency of propofol extraction by ensuring that the extracted amount reflects the original sample concentration, reduces the complexity and cost of the apparatus, and enables direct injection of whole blood samples, enhancing the reliability and precision of analyte measurement in clinical and point-of-care settings.
Implementation Method 1
a lysing agent, said lysing agent having an osmotic pressure lower than the osmotic pressure of the cellular material
Implementation Method 2
a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol
Data Source
AI summary
Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.