Protease Variant for Dishwashing Detergent Stability
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Solution Overview
Problem
Conventional automatic dishwashing detergents with proteases often perform optimally in specific conditions, such as hot long cycles or cold short cycles, but not across a range of programs, due to temperature sensitivity and kinetic rate limitations.
Innovation Solution
A phosphate-free automatic dishwashing detergent composition incorporating a specific protease variant with high sequence identity to reference proteases, such as B. gibsonii-clade subtilisin Bgi02446 or B. lentus subtilisin, which maintains performance across various cycle conditions, including hot long and cold short cycles, by optimizing amino acid substitutions for enhanced stability and activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If proteases are designed to withstand high temperatures, then temperature robustness is improved, but kinetic rate becomes slow due to structural rigidity
Solution Approach 1:
The patent applies parameter changes by modifying amino acid residues in the protease structure (e.g., positions 99, 116, 126, 127, 128, 195, 207, 211, 212) to optimize the balance between thermal stability and catalytic activity. These structural parameter modifications enable the protease to maintain both high temperature robustness and fast kinetic rate simultaneously.
Solution Approach 2:
The invention creates a composite enzyme system by combining multiple protease variants with different amino acid substitutions into a single detergent composition. This allows the formulation to leverage the complementary strengths of different protease variants, achieving both thermal stability and high kinetic rate across various washing conditions.
2Reliability
If a composition is designed to have optimum performance in a hot long cycle, then cleaning performance in hot long cycles is improved, but performance in cold short cycles deteriorates
Solution Approach 1:
The patent achieves universality by formulating a detergent composition containing multiple protease variants that collectively provide optimal cleaning performance across diverse washing conditions. Each protease variant is optimized for specific conditions, and their combination creates a multi-functional system that adapts to different cycle types including hot long, cold short, and intermediate programs.
Solution Approach 2:
The invention applies dynamics by creating a protease system where different enzyme variants become increasingly active under different temperature and time conditions. The composition dynamically adapts to various cycle parameters, with proteases activating at different rates and intensities depending on the specific washing conditions, ensuring consistent performance across all programs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition provides improved cleaning performance across a wide range of dishwasher programs, ensuring effective removal of food residue even in heavily soiled conditions, regardless of cycle duration or temperature, while being environmentally friendly by being phosphate-free.
Implementation Method 1
The composition comprises a specific protease... Proteases can be susceptible to temperature... Proteases designed to withstand high temperatures usually have a slow kinetic rate
Data Source
AI summary
A phosphate-free automatic dishwashing cleaning composition comprising a protease wherein the protease has at least 80%, preferably at least 85%, more preferably at least 90% and especially at least 96% identity with the amino acid sequence of SEQ ID NO:1 or with the amino acid sequence of SEQ ID NO:2 and wherein the protease comprises amino acid substitutions selected from the group consisting of:(i) at least two amino acid substitutions selected from the group consisting of: X198G/A/K/L/Q/R/T/V/S/L, X207Q, X211Q/N and X212Q in combination with at least three amino acid substitutions selected from the group consisting of: X039E, X074D, X099R, X126A, X127E and X128G; or(ii) X039E-X074D-X099R-X116R-X126A-X127E-X128G-X211Q; X039E-X074D-X099R-X126A-X127E-X128G-X211N; X039E-X074D-X099R-X126A-X127E-X128G-X211Q; X039E-X074D-X099R-X126A-X127E-X128G-X207Q; or(iii) any of the proteases of (i) and (ii) further comprising at least one amino acid substitution selected from X242D and X256E; or(iv) X039E-X074D-X099R-X126A-X127E-X128G-X256E;using the SEQ ID NO:2 numbering.