Protease Variants for Low-Temperature Detergent Stability
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Solution Overview
Problem
Current protease variants used in detergents lack improved stability and performance, particularly at low temperatures and in the presence of detergent ingredients like bleach and surfactants, limiting their effectiveness in cleaning processes.
Innovation Solution
Development of novel protease variants with specific amino acid substitutions that enhance stability and activity, including modifications at positions such as 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19, 22, 23, 24, 27, 28, 30, 39, 40, 42, 43, 44, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 69, 71, 77, 80, 81, 83, 84, 85, 87, 88, 90, 91, 92, 94, 95, 97, 98, 99, 100, 101, 102, 103, 107, 110, 111, 113, 114, 116, 117, 118, 119, 121, 122, 123, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 143, 144, 145, 147, 148, 149, 150, 151, 152, 153, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 168, 169, 174, 176, 177, 178, 182, 183, 184, 185, 186, 187, 188, 189, 191, 192, 193, 194, 196, 197, 198, 199, 200, 202, 203, 204, 206, 209, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 226, 227, 230, 231, 333, 334, 335, 236, 238, 239, 240, 242, 243, 244, 246, 247, 248, 256, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 273, 274, 276, 277, 279, 280, 281, 282, 283, 284, 285, 286, 287, 290, 291, 293, 296, 297, 298, 299, 300, 301, 302, 308, 309, 310, and 311, resulting in improved wash performance and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional protease variants are used in detergent formulations, then basic cleaning function is provided, but stability and performance at low temperatures and in the presence of detergent ingredients are insufficient
Solution Approach 1:
The patent applies parameter changes by systematically modifying amino acid positions in the protease sequence to alter its physical and chemical properties. Specific substitutions at positions such as 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19, 22, 23, 24, 27, 28, 30, 39, 40, 42, 43, 44, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 69, 71, 77, 80, 81, 83, 84, 85, 87, 88, 90, 91, 92, 94, 95, 97, 98, 99, 100, 101, 102, 103, 107, 110, 111, 113, 114, 116, 117, 118, 119, 121, 122, 123, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 143, 144, 145, 147, 148, 149, 150, 151, 152, 153, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 168, 169, 174, 176, 177, 178, 182, 183, 184, 185, 186, 187, 188, 189, 191, 192, 193, 194, 196, 197, 198, 199, 200, 202, 203, 204, 206, 209, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 226, 227, 230, 231, 333, 334, 335, 236, 238, 239, 240, 242, 243, 244, 246, 247, 248, 256, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 273, 274, 276, 277, 279, 280, 281, 282, 283, 284, 285, 286, 287, 290, 291, 293, 296, 297, 298, 299, 300, 301, 302, 308, 309, 310, and 311 create variants with enhanced stability and low-temperature activity while maintaining compatibility with detergent ingredients
Solution Approach 2:
The patent applies local quality by making specific localized amino acid substitutions at defined positions in the protease sequence rather than uniform modifications. Each substitution at specific positions (e.g., positions 2-10, 16-30, 39-50, etc.) is designed to locally alter protein structure and function, creating regions with enhanced stability or activity while preserving overall protein function and compatibility with detergent formulations
2Temperature
If protease variants with improved low-temperature activity are developed, then wash performance at low temperatures is enhanced, but stability in the presence of detergent ingredients like bleach and surfactants may be compromised
Solution Approach 1:
The patent simultaneously optimizes multiple parameters by selecting specific amino acid substitutions that collectively enhance low-temperature activity while maintaining detergent stability. The coordinated modifications at multiple positions (including but not limited to positions 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19, 22, 23, 24, 27, 28, 30, 39, 40, 42, 43, 44, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 69, 71, 77, 80, 81, 83, 84, 85, 87, 88, 90, 91, 92, 94, 95, 97, 98, 99, 100, 101, 102, 103, 107, 110, 111, 113, 114, 116, 117, 118, 119, 121, 122, 123, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 143, 144, 145, 147, 148, 149, 150, 151, 152, 153, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 168, 169, 174, 176, 177, 178, 182, 183, 184, 185, 186, 187, 188, 189, 191, 192, 193, 194, 196, 197, 198, 199, 200, 202, 203, 204, 206, 209, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 226, 227, 230, 231, 333, 334, 335, 236, 238, 239, 240, 242, 243, 244, 246, 247, 248, 256, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 273, 274, 276, 277, 279, 280, 281, 282, 283, 284, 285, 286, 287, 290, 291, 293, 296, 297, 298, 299, 300, 301, 302, 308, 309, 310, and 311) create a balanced profile where the enzyme remains stable in detergent ingredients while maintaining high activity at low temperatures
3Reliability
If protease variants with enhanced detergent stability are created, then stability in the presence of bleach and surfactants is improved, but activity at low temperatures may be reduced
Solution Approach 1:
The patent achieves simultaneous improvement in detergent stability and low-temperature activity through coordinated amino acid substitutions at multiple positions. The specific combinations of substitutions at positions such as 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19, 22, 23, 24, 27, 28, 30, 39, 40, 42, 43, 44, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 69, 71, 77, 80, 81, 83, 84, 85, 87, 88, 90, 91, 92, 94, 95, 97, 98, 99, 100, 101, 102, 103, 107, 110, 111, 113, 114, 116, 117, 118, 119, 121, 122, 123, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 143, 144, 145, 147, 148, 149, 150, 151, 152, 153, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 168, 169, 174, 176, 177, 178, 182, 183, 184, 185, 186, 187, 188, 189, 191, 192, 193, 194, 196, 197, 198, 199, 200, 202, 203, 204, 206, 209, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 226, 227, 230, 231, 333, 334, 335, 236, 238, 239, 240, 242, 243, 244, 246, 247, 248, 256, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 273, 274, 276, 277, 279, 280, 281, 282, 283, 284, 285, 286, 287, 290, 291, 293, 296, 297, 298, 299, 300, 301, 302, 308, 309, 310, and 311) are selected to maintain the enzyme's catalytic efficiency at low temperatures while enhancing its resistance to detergent ingredients through structural stabilization
Data Source
AI summary
The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.