Protease Dishwashing Pouch Composition for Oxidative Stability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
There is a need for improved protease variants with enhanced stability in oxidative environments, particularly in automatic dishwashing compositions, and for a dishwashing detergent composition that provides good cleaning performance and stability, including a water-soluble unit-dose pouch with both powder and gel components.
Innovation Solution
An automatic dishwashing composition comprising a surfactant and a protease variant with specific mutations, such as at position 122 and one or more mutations at positions 126, 127, 128, 211, and 212, packaged in a water-soluble unit-dose pouch with a powder and gel component, and optionally containing bleach, to enhance cleaning and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a protease is used in combination with bleach in automatic dishwashing composition, then cleaning performance is improved, but protease stability deteriorates due to oxidative environments
Solution Approach 1:
The patent applies parameter changes by introducing specific amino acid mutations at positions 122, 126, 127, 128, 211, and 212 of the subtilisin variant. These mutations alter the molecular structure and chemical properties of the protease, enhancing its resistance to oxidative degradation by bleach while preserving its proteolytic activity for effective cleaning
Solution Approach 2:
The patent creates a composite enzyme system by combining multiple mutated subtilisin variants with specific amino acid substitutions. This composite approach synergistically improves oxidative stability while maintaining cleaning efficacy, as each mutation contributes to different aspects of stability and activity
2Reliability
If a water-soluble unit-dose pouch with both powder and gel components is used, then cleaning performance and stability are improved, but device complexity increases
Solution Approach 1:
The patent divides the detergent composition into distinct segments: a powder component containing builders and surfactants, and a gel component containing the protease variant. This segmentation prevents premature interaction between components, maintaining stability during storage while ensuring effective cleaning during use
Solution Approach 2:
The patent employs a nested structure where the gel component containing the protease is enclosed within or alongside the powder component in a water-soluble pouch. This nested arrangement protects the sensitive protease from oxidative damage by bleach until dissolution in the dishwasher, simplifying the overall system while maintaining performance
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition achieves effective cleaning performance and stability, even in oxidative environments, with a compact and stable form suitable for dishwasher use.
Implementation Method 1
A protease has the ability to conduct proteolysis, which begins protein catabolism by hydrolysis of peptide bonds that link amino acids together in a peptide or polypeptide chain
Implementation Method 2
automatic dishwashing composition in the form of a water-soluble unit-dose pouch
Data Source
AI summary
Fabric and home care compositions including a surfactant and a protease. The automatic dishwashing composition is in the form of a water-soluble unit-dose pouch including a compartment. The compartment includes both a powder component and a gel component. The protease is a subtilisin variant that includes: (i) a mutation at position 122; and (ii) one or more mutations at positions 126, 127, 128, 211 and 212, with reference to SEQ ID NO: 1. The variant has at least about 80% identity to the amino acid sequence of SEQ ID NO: 1.