Protease Variants for Detergent Stability
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Solution Overview
Problem
Current detergent compositions using proteases face challenges in achieving improved wash performance and stability, particularly in low temperatures and the presence of detergents like bleach and surfactants, where existing proteases may lose activity.
Innovation Solution
Development of protease variants with specific amino acid substitutions at positions 173, 171, 175, 179, or 180, maintaining at least 70% sequence identity to the mature polypeptide, which enhance stability and wash performance by improving the S1 substrate binding pocket's functionality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If protease is used in detergent composition, then wash performance is improved, but stability in challenging conditions (low temperature, bleach, surfactants) deteriorates
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the protease at specific positions (173, 171, 175, 179, 180) to alter its physical and chemical properties. These sequence modifications change the enzyme's stability parameters without significantly affecting its catalytic activity, allowing it to maintain wash performance while improving stability in challenging detergent conditions including low temperature, bleach, and surfactant presence
2Reliability
If amino acid substitutions are introduced to improve stability, then detergent stability is improved, but protease activity may be reduced
Solution Approach 1:
The patent applies local quality by introducing amino acid substitutions at specific local positions (173, 171, 175, 179, 180) within the protease sequence rather than throughout the entire molecule. These localized changes specifically target regions that influence stability without disrupting the catalytic core, thereby improving detergent stability while preserving protease activity
Solution Approach 2:
The patent carefully selects amino acid substitutions that change stability parameters while maintaining activity parameters. By modifying specific residues at defined positions and controlling the degree of sequence identity (at least 70% to the mature polypeptide), the invention achieves improved stability without significant loss of protease activity
Data Source
Figure 1
AI summary
The present invention relates to detergent compositions comprising protease variants and methods for obtaining such detergent compositions. The present invention also relates to the use of such detergent compositions, especially in laundry or in hard surface cleaning applications.