Modified Protease Variants for Low-Temperature Stain Removal
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Solution Overview
Problem
Current proteases used in detergents and cleaning agents are not optimally effective for removing protease-sensitive stains, particularly at low temperatures and on specific stains like blood and whole egg, due to limitations in their alkaline pH optimum and stability.
Innovation Solution
Development of a protease with an amino acid sequence identical to that of Bacillus gibsonii DSM14391 or Bacillus sp. DSM14392, with specific substitutions such as N97D, S97D, R99E, or S99E, which enhances its cleaning performance on protease-sensitive stains by maintaining activity across a wide temperature range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current proteases are used in detergents, then they can break down protein-containing stains, but they are not optimally effective at low temperatures and on specific stains like blood and whole egg
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the protease (specifically positions 97 and 99) to alter its functional properties. This enables the enzyme to maintain optimal activity across a broader temperature range, particularly improving performance at low temperatures while retaining effectiveness on protease-sensitive stains like blood and whole egg.
2Productivity
If proteases with modified amino acid sequences are developed, then cleaning performance is improved, but the complexity of enzyme development increases
Solution Approach 1:
The patent applies local quality by making targeted, localized modifications to specific amino acid positions (97 and 99) in the protease sequence rather than进行全面改造. This focused approach improves cleaning performance while minimizing the overall complexity of enzyme development and characterization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified protease demonstrates improved cleaning performance on stains like blood and whole egg, even at low temperatures, and maintains proteolytic activity, enhancing the efficacy of detergents and cleaning agents.
Implementation Method 1
They act as unspecific endopeptidases and hydrolyze any acid amide bonds located inside peptides or proteins
Implementation Method 2
They cause the breakdown of protein-containing stains from the item to be cleaned
Data Source
AI summary
The disclosure relates to proteases, the amino acid sequence of which has been modified in particular with regard to the use thereof in detergents and cleaning agents, all sufficiently similar proteases having a corresponding modification and nucleic acids coding for them. The disclosure further relates to methods and uses of said proteases and to agents containing them, in particular detergents and cleaning agents.