Protein Isolate Production via Enzymatic Fiber Removal
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Solution Overview
Problem
Defatted oilseed meals contain high amounts of fiber and anti-nutritional factors, making them unattractive for commercial use due to the difficulty in separating proteins from these components, especially with high salt content solutions that are uneconomical to recover.
Innovation Solution
A process involving heat treatment, enzyme treatment with cellulase and phytase, and solvent extraction to remove fiber and anti-nutritional factors, resulting in protein concentrates and isolates with high protein content, including steps like mixing with solvents, enzyme addition, pH adjustment, and solvent washing to separate and purify protein fractions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If high salt content aqueous solution is used to dissolve canola protein, then protein separation from fiber is improved, but salt removal and recovery becomes difficult and uneconomical
Solution Approach 1:
The patent changes the solvent parameter from high salt content aqueous solution to low salt or salt-free aqueous solution, maintaining protein solubility while eliminating the salt removal problem. This parameter change resolves the contradiction by achieving effective protein-fiber separation without introducing the salt recovery difficulty.
Solution Approach 2:
The patent introduces an intermediary substance (low salt or salt-free aqueous solution) that facilitates protein dissolution and separation from fiber without creating the salt removal problem. This intermediary solvent acts as a mediator between the protein and fiber, enabling separation while avoiding the harmful salt recovery issue.
2Quantity of substance
If defatted meal with high fiber content is used directly, then production cost is reduced, but commercial usability deteriorates due to anti-nutritional factors
Solution Approach 1:
The patent extracts and removes fiber and anti-nutritional factors from the defatted meal through dissolution and filtration processes. This extraction removes the harmful components while retaining the valuable protein, resolving the contradiction between maintaining low production cost and achieving high commercial usability.
Solution Approach 2:
The patent changes the purity parameter of the protein product by removing fiber and anti-nutritional factors, transforming the defatted meal from an unsuitable raw material into a high-quality protein concentrate or isolate. This parameter change enables commercial usability while maintaining cost-effectiveness.
3Manufacturing precision
If extensive processing is applied to remove fiber and anti-nutritional factors, then product quality is improved, but production complexity increases
Solution Approach 1:
The patent merges multiple processing functions into a unified dissolution-filtration system. By combining protein dissolution, fiber separation, and anti-nutritional factor removal into a single aqueous extraction and filtration process, the patent achieves high product quality while minimizing processing complexity.
Solution Approach 2:
The patent employs a universal aqueous extraction process that simultaneously dissolves protein, separates fiber, and removes anti-nutritional factors. This multi-functional approach achieves comprehensive purification in a single process stream, resolving the contradiction between product quality and processing complexity.
Data Source
AI summary
Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from an oilseed meal using low g-force centrifugation.