Proteoglycan Extraction Using Physiological Salts Instead of EDTA
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Solution Overview
Problem
Existing methods for producing proteoglycan are inefficient due to the use of harmful substances such as EDTA, and the production process is complicated to remove the harmful substances.
Innovation Solution
A method for producing proteoglycan. The method includes extracting a composition of the present disclosure provides a method for producing proteoglycan. The method includes extracting a composition of the present disclosure provides a method for producing proteoglycan. The method includes extracting a composition of the present disclosure provides a method for producing proteoglycan.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If known methods for producing proteoglycan are used, then proteoglycan can be produced, but harmful substances such as EDTA must be used and the production process becomes complicated
Solution Approach 1:
The patent extracts and eliminates harmful substances (EDTA and other chelating agents) from the proteoglycan production process. The invention achieves this by using a simplified extraction method that relies on natural proteoglycan solubility properties rather than chemical chelators, thereby removing harmful factors while maintaining production capability
Solution Approach 2:
The patent changes the chemical parameters of the production process by replacing harmful chelating agents with physiological salt solutions. This parameter change transforms the production method from one requiring complex chemical agents to one using benign physiological conditions, simplifying the process and eliminating harmful substances
2Productivity
If known methods for producing proteoglycan are used, then proteoglycan production can proceed, but the yield of proteoglycan is reduced
Solution Approach 1:
The patent segments the production process into distinct stages: tissue preparation, extraction using physiological salts, and purification. This segmentation allows each stage to be optimized independently, improving overall yield while maintaining manageable process complexity through clear separation of functions
Solution Approach 2:
The patent maintains continuous proteoglycan extraction and preservation throughout the process by using physiological salt solutions that prevent degradation. This continuous protective action ensures high yield by minimizing loss at each process transition, avoiding the need for complex intermediate processing steps
3Productivity
If harmful substances are used in proteoglycan production, then extraction can be effective, but additional processing steps are required to remove harmful substances
Solution Approach 1:
The patent converts the traditional approach of using harmful substances to achieve extraction into a beneficial process by using physiological salt solutions. This inversion transforms what would be a harmful chemical process into a benign physiological extraction, eliminating the need for subsequent harmful substance removal steps and saving time
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for producing proteoglycan in a simpler manner than known methods, and produces proteoglycan with a core protein and sugar chains bonded.
Implementation Method 1
extracting an extract comprising proteoglycan from a tissue of an animal
Data Source
AI summary
The present disclosure produces proteoglycan in a simpler manner than known methods for producing proteoglycan, and provides a method capable of producing proteoglycan with a core protein and sugar chains bonded. The method for producing proteoglycan of the present disclosure includes: extracting an extract c proteoglycan from a tissue of an animal. The proteoglycan comprises a core protein and a sugar chain, and the core protein and the sugar chain are bonded.