Platelet-Rich Plasma Preparation Using Thixotropic Gel Separation
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Solution Overview
Problem
Existing methods for preparing platelet-rich plasma (PRP) are complex, costly, and inefficient, leading to significant loss of valuable biological tissue, and cryoconservation techniques alter cell viability and stability, limiting their use for extemporaneous applications in tissue regeneration.
Innovation Solution
A simplified process using specific separator tubes with thixotropic gels and controlled centrifugation to produce a platelet concentrate with high yields, combined with a coagulation activator and optional cell extracts, ensuring high concentrations of platelets, white blood cells, and fibrinogen, suitable for immediate use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If current methods for preparing platelet-rich plasma are used, then platelet concentration is achieved, but the process becomes complex and costly with significant loss of valuable biological tissue
Solution Approach 1:
The invention extracts only the necessary components (platelets and plasma) from whole blood using a specialized centrifugation process with thixotropic gel barriers, separating them from unnecessary components (red blood cells, white blood cells) to minimize loss of valuable biological tissue while achieving high platelet concentration
Solution Approach 2:
The invention changes the physical parameters of the separation process by using thixotropic gels that change viscosity under centrifugal force, creating dynamic barriers that improve separation efficiency and reduce tissue loss while maintaining high platelet yield
2Quantity of substance
If current methods for preparing platelet-rich plasma are used, then platelet enrichment is achieved, but variability in yield increases and reliability decreases
Solution Approach 1:
The invention introduces thixotropic gel barriers as intermediaries in the centrifugation process that standardize the separation mechanism, creating consistent and reproducible platelet enrichment results by mediating the interaction between blood components during centrifugation
Solution Approach 2:
The invention standardizes the centrifugation parameters and gel composition to create a reliable, repeatable process that reduces yield variability while maintaining high platelet enrichment levels
3Duration of action of stationary object
If platelet concentrates are prepared for long-term conservation, then storage is enabled, but cell viability and stability are altered
Solution Approach 1:
The invention performs preliminary enrichment and concentration of platelets before storage, creating a more stable concentrate that maintains viability and growth factor activity during storage by pre-establishing optimal conditions in the concentrate
4Quantity of substance
If complex preparation methods are used, then platelet concentration is achieved, but device complexity and cost increase
Solution Approach 1:
The invention segments the blood components into distinct layers using thixotropic gel barriers during centrifugation, allowing for simple, automated collection of the platelet-rich plasma layer without complex processing equipment
Solution Approach 2:
The invention uses disposable separator tubes with integrated thixotropic gel barriers that simplify the preparation process and eliminate the need for complex, expensive equipment while achieving high platelet concentration
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process achieves a reliable, cost-effective preparation of platelet concentrates with enhanced growth factor secretion and viability, enabling effective wound healing, tissue regeneration, and cosmetic applications without the drawbacks of previous methods.
Implementation Method 1
centrifuging whole blood in a separator tube... to form a barrier between the plasma containing the platelets, the lymphocytes and the monocytes and the pellet containing the erythrocytes
Implementation Method 2
a glass separator tube containing a polyester-based thixotropic gel
Data Source
AI summary
The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.