PRP Isolation Kit Filtration for Leukocyte-Free Platelet Separation

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Solution Overview

Problem

Existing commercial PRP kits face challenges in efficiently separating platelets from white and red blood cells, leading to contamination and inflammation, which affects the quality and efficacy of platelet-rich plasma for tissue repair.

Innovation Solution

A two-step process using a first isolation tube with a filter and a second isolation tube to separate buffy coat and plasma layers, followed by centrifugation to collect platelet-rich plasma, effectively removing leukocytes and erythrocytes through a filter with pore sizes ranging from 1 to 5 μm.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If traditional centrifugation is used to separate platelets from whole blood, then platelet concentration is improved, but leukocyte and erythrocyte contamination increases

Engineering Contradiction:

Improveplatelet concentrationVSAvoidleukocyte and erythrocyte contamination

Core Design Contradiction:

Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent employs a filter membrane with specific pore size (1-5 μm) that allows plasma and platelets to pass through while blocking larger leukocytes and erythrocytes. This porous material approach resolves the contradiction by enabling selective separation based on cell size, achieving high platelet concentration without leukocyte contamination.

Inventive Principle:

Principle #31Porous materials

Solution Approach 2:

The invention extracts the harmful components (leukocytes and erythrocytes) from the blood sample by filtering them out through the isolation tube system. The filter selectively removes these contaminants while retaining platelets in the plasma, thus resolving the contamination issue while maintaining platelet concentration.

Inventive Principle:

Principle #2Taking out (Extraction)

2Quantity of substance

If buffy-coat based kits are used to harvest platelets, then platelet concentration is improved, but fibrin ultra-structure formation is compromised

Engineering Contradiction:

Improveplatelet concentrationVSAvoidfibrin ultra-structure

Core Design Contradiction:

Quantity of substanceVSManufacturing precision

Solution Approach 1:

The patent changes the separation parameters by using a filter-based approach rather than density-based centrifugation. This parameter change ensures that platelets are isolated in a way that preserves their functional properties and ability to form proper fibrin ultra-structure, while still achieving high concentration.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The filter membrane with controlled pore size allows for gentle separation that maintains platelet integrity and fibrinogen functionality. This enables proper fibrin ultra-structure formation while achieving high platelet concentration in the isolated PRP.

Inventive Principle:

Principle #31Porous materials

3Volume of moving object

If plasma-based kits are used to collect platelets, then plasma volume is improved, but platelet number decreases

Engineering Contradiction:

Improveplasma volumeVSAvoidplatelet number

Core Design Contradiction:

Volume of moving objectVSQuantity of substance

Solution Approach 1:

The invention adds a spatial dimension to separation by using a filter membrane that creates size-based zones within the plasma. This allows platelets to be concentrated in specific regions of the isolated plasma, achieving both high plasma volume and high platelet number simultaneously.

Inventive Principle:

Principle #17Another dimension (Dimensionality change)

Solution Approach 2:

The filter membrane acts as an intermediary that enables the coexistence of large plasma volume and high platelet concentration. It mediates the separation process to produce PRP with both adequate plasma volume for delivery and sufficient platelet number for therapeutic effect.

Inventive Principle:

Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method achieves high platelet concentration and purity in PRP, reducing leukocyte and erythrocyte contamination, thereby enhancing the healing process by maintaining stable growth factor release.

Implementation Method 1

a filter with a pore size in a range of 1.5 to 4 μm

Methodology Applied

Scientific EffectFiltration: Filter (physical)

Implementation Method 2

centrifugation process as the conventional way brings the whole blood into three layers

Methodology Applied

Scientific EffectCentrifugal separation: Centrifugal Separation

Data Source

PatentUS12564833B2Kit for isolation of platelet-rich plasma and the method using the same

Publication Date: 2026.03.03 KARTIGEN BIOMEDICAL INC

AI summary

Provided herein is a kit for isolation of platelet-rich plasma, comprising a first isolation tube and a second isolation tube. The first isolation tube comprises a first opening and a second opening, and the second isolation tube comprises a first portion, a second portion, and a filter, wherein the first portion has a third opening, the filter is disposed between the first portion and the second portion, the second opening has a diameter in a range of 1 to 5 mm and the filter has a pore size in a range of 1.5 to 4 μm.