PRP and Stem Cell Medium Composite for Accelerated Wound Healing
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current wound healing compositions, including those using platelet enriched plasma (PRP) and stem cell conditioned medium (SC CM), have limitations in efficiency and speed, with typical healing times ranging from 3 to 6 weeks, necessitating the development of alternative compositions that can accelerate the healing process.
Innovation Solution
A composition comprising platelet enriched plasma (PRP) and stem cell conditioned medium (SC CM), where SC CM is harvested after culturing mesenchymal stem cells, combined with DMSO and a gelling agent, applied in a ratio of 3:1 to 1:3, to enhance wound healing by promoting angiogenesis, collagen synthesis, and tissue regeneration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If conventional wound healing compositions (PRP or SC CM alone) are used, then wound healing occurs, but the healing time is prolonged (3 to 6 weeks)
Solution Approach 1:
The patent combines platelet enriched plasma (PRP) and stem cell conditioned medium (SC CM) into a single composite composition. PRP provides growth factors like PDGF, TGF-β, and VEGF, while SC CM contributes hepatocyte growth factor, keratinocyte growth factor, and other cytokines. This merging of two complementary biological products creates a synergistic effect that accelerates wound healing to 1-2 weeks, resolving the contradiction between healing time and efficiency.
2Productivity
If PRP and SC CM are combined in optimal ratios, then wound healing efficiency is maximized, but the complexity of composition preparation increases
Solution Approach 1:
The patent specifies optimal ratio ranges for PRP to SC CM (from 1:4 to 4:1) and controlled incubation temperatures (37°C ± 5°C) with time periods of 24-72 hours. By defining these parameter ranges, the patent simplifies the preparation process while maintaining high healing efficiency, resolving the contradiction between productivity and preparation complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The combination significantly reduces wound healing time to 1 to 2 weeks, providing fast and effective cutaneous wound healing, promoting hair growth, and reducing inflammation, with 100% biocompatibility and safety.
Implementation Method 1
During proliferation, the wound is 'rebuilt' with new granulation tissue which is comprised of collagen and extracellular matrix and into which a new network of blood vessels develop, a process known as 'angiogenesis'
Implementation Method 2
During proliferation, the wound is 'rebuilt' with new granulation tissue which is comprised of collagen and extracellular matrix
Data Source
Figure 1A~2B
Figure 3A~4B
Figure 5A~6B
AI summary
The present invention relates to compositions for use in pharmaceutical, cosmetic and cosmeceutical applications, particularly wound healing, including the treatment of lesions and burns. In particular, the present invention relates to compositions comprising platelet enriched plasma (PRP) and stem cell conditioned medium (SC CM) for use in pharmaceutical, cosmetic and cosmeceutical applications such as wound healing.