PRRSV Detection via N Protein Binding in Biological Fluids
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Solution Overview
Problem
Current methods for detecting porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) are not consistently effective, leading to ongoing economic losses in the swine industry, as existing vaccines do not reliably eradicate the disease, and detection methods are costly and dependent on tissue samples.
Innovation Solution
A method and device using an agent that binds the nucleocapsid (N) protein of PRRSV, allowing for detection in biological fluids, employing a binding agent such as a monoclonal antibody that forms a complex with the N protein, which can be immobilized and labeled for easy detection, facilitating rapid diagnosis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If tissue samples are used for PRRSV detection, then detection accuracy is improved, but operation complexity and cost increase
Solution Approach 1:
The invention extracts the detection target from tissue samples to biological fluids. By detecting PRRSV N protein in readily obtainable biological fluids like saliva and nasal discharge instead of requiring tissue biopsies, the method maintains detection accuracy while dramatically simplifying the sampling process and reducing operational complexity
Solution Approach 2:
The invention uses an antibody-specific binding agent as an intermediary to detect PRRSV N protein in biological fluids. This binding agent mediates between the virus protein and the detection system, enabling accurate detection through simple fluid sampling without direct tissue analysis
2Reliability
If conventional detection methods are used, then detection capability is maintained, but time consumption and cost increase
Solution Approach 1:
The invention segments the detection process into simple, rapid steps: collect biological fluid, mix with binding agent, and read results. This segmentation eliminates time-consuming steps like tissue processing and complex laboratory procedures, reducing detection time while maintaining reliability through the specific antibody-based detection method
Solution Approach 2:
The invention employs a disposable rapid test device format that eliminates the need for expensive, time-consuming laboratory equipment and complex processing procedures. The single-use nature of the device maintains detection reliability while dramatically reducing both time consumption and operational cost
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection of PRRSV infection in biological fluids, providing a rapid and cost-effective means to diagnose the presence of the virus, reducing reliance on tissue samples and improving eradication efforts.
Implementation Method 1
The invention provides a binding agent capable of binding N protein from PRRSV in a sample of a biological fluid
Data Source
AI summary
The invention provides methods, devices, and kits for the detection of a class of porcine reproductive and respiratory syndrome (PRRS) virus, or PRRSV, in a biological fluid. The invention is based in part on an agent which binds the nucleocapsid (N) protein of PRRSV. The agent may be an antibody that binds the N protein with specificity. Compositions and devices comprising the binding agent, as well as methods of using it are also provided. Also provided are methods of preparing devices and kits for the practice of the methods.