Pseudomonas sp. CECT8708 Biocontrol via Siderophore Iron Sequestration
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Solution Overview
Problem
Chemical pesticides contaminate the environment, pose health risks, and have variable effectiveness due to host and environmental conditions, necessitating the development of more effective and environmentally friendly biocontrol agents.
Innovation Solution
A novel strain of Pseudomonas sp. (CECT8708) with antibacterial and antifungal properties, producing siderophores and surfactants, demonstrating high efficacy against pathogens and environmental stability, and being resistant to certain antibiotics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical pesticides are used to control plant diseases, then pest control effectiveness is improved, but environmental contamination and health risks increase
Solution Approach 1:
The patent replaces chemical pesticides with a biological control system using Pseudomonas sp. strain CECT8708. The bacterium produces siderophores that sequester iron from the environment, depriving fungal pathogens of this essential nutrient and thereby controlling plant diseases through a biological mechanism rather than chemical toxicity.
Solution Approach 2:
The patent introduces siderophores as intermediary substances produced by the Pseudomonas sp. strain. These siderophores act as mediators that indirectly control fungal growth by competing for iron resources, rather than directly attacking the pathogen with chemical pesticides.
2Object-affected harmful factors
If conventional biocontrol agents are used, then environmental safety is improved, but effectiveness is reduced due to variable biological activity under different host and environmental conditions
Solution Approach 1:
The patent identifies and exploits specific physiological parameters of the Pseudomonas sp. strain CECT8708, including its optimal growth temperature range (28-30°C) and its ability to produce siderophores under iron-limited conditions. By understanding and optimizing these parameters, the biocontrol agent maintains consistent effectiveness across varying environmental conditions.
Solution Approach 2:
The patent employs a readily cultivable bacterial strain that can be produced cost-effectively and applied as a living biocontrol agent. The strain's ability to rapidly reproduce and adapt to different plant hosts ensures sustained protection without requiring expensive or complex formulations.
3Object-affected harmful factors
If biocontrol agents are developed to replace chemical pesticides, then environmental friendliness is improved, but variable effectiveness under different conditions reduces reliability
Solution Approach 1:
The Pseudomonas sp. strain CECT8708 is engineered to produce its own siderophores and adapt to different environmental conditions independently. The bacterium's natural ability to sense iron availability and regulate siderophore production ensures consistent biocontrol activity without requiring external inputs or complex formulation adjustments.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Pseudomonas sp. strain effectively inhibits bacterial and fungal infections in plants, outperforming commercial biocontrol agents and maintaining safety for humans and the environment, with a broad temperature and salt tolerance range.
Implementation Method 1
the strain of the invention has the ability to produce and secrete to the medium siderophores and surfactant metabolites
Implementation Method 2
the strain of the invention has surfactant activity which, on one hand, contributes to the high anti-bacterial and fungal activity of the strain of the invention
Data Source
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AI summary
The present invention relates to a new isolated strain of Pseudomonas sp. deposited at the "Colección Española de Cultivos Tipo" (CECT) under the accession number CECT8708 or a mutant thereof; wherein said mutant is obtained using the CECT8708 of Pseudomonas sp. as starting material, maintains the dual bactericidal and fungicidal effects of CECT8708 and has a genome with an Average Nucleotide Identity (ANI) to the genome sequence SEQ ID NO: 1 of at least 99.8%., The strain of the invention has bactericidal and fungicidal effect. The invention also relates to the inoculation product comprising the strain of the invention, the cell-free extract derived from the strain of the invention, and the composition comprising it and their uses in the control of bacteria and/or fungal infection in a plant. Also refers to a method to obtain the mutant strain of the strain of the invention.