Psicose Purification via Mannitol Dehydrogenase Conversion
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Solution Overview
Problem
Conventional methods for producing psicose are inefficient due to high costs and low production capacity, and face challenges in separating psicose from fructose due to their similar physical properties.
Innovation Solution
A method involving the use of mannitol dehydrogenase to convert fructose into mannitol, allowing for easier isolation of psicose, which is achieved by reacting a mixture of fructose and psicose with mannitol dehydrogenase, utilizing specific epimerases such as Agrobacterium tumefaciens- or Anaerostipes caccae-derived psicose-3-epimerase, and culturing microorganisms like Corynebacterium glutamicum to enhance thermal stability and production efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional chemical methods or biological methods are used to produce psicose from fructose, then psicose can be produced, but the production efficiency is low and the cost is high
Solution Approach 1:
The patent changes the chemical parameter of the reaction system by using a specific enzyme (psicose-3-epimerase) from Agrobacterium tumefaciens with optimized reaction conditions (pH 7.0-8.0, temperature 25-37°C) to achieve high conversion efficiency (over 90%) while reducing production costs compared to conventional chemical catalysts or other biological methods
Solution Approach 2:
The patent introduces psicose-3-epimerase as an intermediary substance that catalyzes the conversion of fructose to psicose. This enzyme acts as a biological mediator that enables efficient transformation without the need for expensive chemical catalysts or complex multi-step biological processes
2Manufacturing precision
If psicose is produced from fructose using conventional methods, then psicose can be obtained, but separation of psicose from fructose is difficult due to similar physical properties
Solution Approach 1:
The patent extracts and utilizes the specific biological property of psicose-3-epimerase that shows high substrate specificity for fructose conversion to psicose. By using this enzyme's inherent selectivity, the method effectively 'extracts' psicose from the mixture through selective conversion, avoiding complex physical separation processes
Solution Approach 2:
Instead of attempting to separate psicose from fructose directly, the patent inverts the approach by selectively converting remaining fructose to psicose using the epimerase enzyme. This converts the separation problem into a conversion problem, achieving high purity psicose through selective transformation rather than physical separation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method improves psicose production capacity and purity, enabling high-yield and efficient separation of psicose from fructose, thereby addressing the inefficiencies and separation challenges of existing methods.
Implementation Method 1
adding a mannitol dehydrogenase to a mixture of fructose and psicose to convert the fructose into mannitol
Implementation Method 2
producing D-tagatose-3-epimerase of a separated microorganism, for example, Pseudomonas cichorii ST-24, in recombinant E. coli
Data Source
AI summary
A method for obtaining psicose from a mixture of fructose and psicose includes adding a mannitol dehydrogenase to a mixture of fructose and psicose to convert fructose into mannitol, and separating the mannitol from the psicose. Accordingly, the psicose can be more easily isolated from the mannitol with high efficiency by using the method.