Lactiplantibacillus plantarum PTA22 Probiotics for Rabbit Gut Health

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Solution Overview

Problem

Rabbits fed with foods low in crude fiber often experience gastrointestinal disorders due to abnormal bacterial growth, such as Escherichia coli and Clostridium spp., leading to issues like diarrhea and intestinal obstruction, as these foods ferment easily and promote pathogenic bacteria growth.

Innovation Solution

A novel probiotic strain, Lactiplantibacillus plantarum PTA22, with enhanced acid tolerance, colonization ability, and oxalic acid degradation activity, is isolated and formulated into a nutritional composition for rabbits, which includes high biological value proteins, oligosaccharides, and excipients to inhibit pathogenic bacteria and improve digestion.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Use of energy by moving object

If rabbits are fed foods low in crude fiber, then energy availability is improved, but gastrointestinal health deteriorates due to abnormal bacterial growth

Engineering Contradiction:

Improveenergy availabilityVSAvoidgastrointestinal health

Core Design Contradiction:

Use of energy by moving objectVSReliability

Solution Approach 1:

The patent introduces probiotics as intermediary substances that mediate between the low-fiber diet and gastrointestinal health. These probiotics inhibit pathogenic bacteria growth and maintain gut flora balance, allowing rabbits to consume low-fiber foods without suffering from gastrointestinal disorders.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent changes the microbial parameter of the gastrointestinal tract by introducing specific probiotic strains. This parameter change enables the system to tolerate low-fiber diets while maintaining gut health, as the probiotics alter the bacterial composition to prevent pathogenic growth.

Inventive Principle:

Principle #35Parameter changes

2Quantity of substance

If high-carbohydrate foods are fed to rabbits, then energy density is improved, but pathogenic bacteria growth is promoted

Engineering Contradiction:

Improveenergy densityVSAvoidpathogenic bacteria growth

Core Design Contradiction:

Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

Probiotics serve as intermediary agents that counteract the harmful effect of carbohydrates on pathogenic bacteria. The probiotics compete for resources and produce substances that inhibit pathogenic growth, allowing high-carbohydrate foods to be consumed without promoting disease.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent converts the potentially harmful effect of carbohydrates into a beneficial situation by using probiotics to selectively inhibit pathogenic bacteria. The carbohydrates still provide energy, but the probiotic intervention prevents the harmful bacterial growth that would otherwise occur.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

3Reliability

If probiotics are added to food, then gastrointestinal health is improved, but manufacturing complexity increases

Engineering Contradiction:

Improvegastrointestinal healthVSAvoidmanufacturing complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent merges the probiotics with food ingredients or feed materials to create a unified product. This combining approach simplifies manufacturing by integrating multiple functions (nutrition and probiotic delivery) into a single product rather than requiring separate administration systems.

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The probiotic formulation is designed to serve multiple functions: providing gastrointestinal health benefits, enhancing food palatability, and improving nutrient absorption. This multi-functionality reduces the need for separate products and simplifies overall manufacturing and application.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The probiotic composition effectively reduces the risk of gastrointestinal disorders, enhances resistance to pathogenic bacteria, and helps in reducing hypercalciuria and calculus formation by promoting a healthy gut microbiome and degrading oxalic acid.

Implementation Method 1

the probiotic PTA22 has oxalic acid degradation activity

Methodology Applied

Scientific EffectOxalic acid degradation: Enzyme

Implementation Method 2

The ability of acid tolerance, colonization in the gastrointestinal tract and so on of one of the novel probiotics

Methodology Applied

Scientific EffectColonization:

Implementation Method 3

The ability of acid tolerance, colonization in the gastrointestinal tract and so on of one of the novel probiotics

Methodology Applied

Scientific EffectAcid tolerance:

Implementation Method 4

the probiotic PTA22 can inhibit the growth of a pathogenic bacterium comprising at least one of Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella enterica

Methodology Applied

Scientific EffectAntibacterial activity:

Data Source

PatentUS20240390438A1Probiotics separated from rabbit feces and food containing probiotics

Publication Date: 2024.11.28 AGENT INNOVATION CO LTD

AI summary

The present disclosure provides a probiotics of PTA22 from rabbits, a nutritional composition for preparing food of rabbits and a composition for rabbits to degrade oxalic acid. Through this disclosure, the health of rabbits can be ensured and the resistance to pathogenic bacteria can be improved after rabbits consume the food containing PTA22. As well, the probiotic PTA22 can help rabbits reduce the risk of hypercalciuria and calculus.