Pulse Wave Treated Water for Stem Cell Apoptosis Inhibition
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Solution Overview
Problem
Existing pulse treatment technologies for water are limited in application range and do not effectively inhibit apoptosis in stem cells during culture, affecting their proliferation and repair.
Innovation Solution
A method involving pulse waves with specific power and frequency parameters is used to treat water, altering its molecular structure to enhance dissolved oxygen content and beneficial elements while reducing harmful substances, and this treated water is used in stem cell culture media to inhibit apoptosis and promote repair.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional pulse treatment technology is applied to water, then free radical concentration in food is reduced, but the application range is limited and cannot effectively inhibit stem cell apoptosis
Solution Approach 1:
The patent applies parameter changes by optimizing specific pulse wave characteristics including duty ratio (1.3-3.5 μs), pulse intervals (0.7-3.1 μs), power (28W or 35W), and frequency (1.5M or 1.9M) to transform conventional pulse treatment into an effective stem cell culture medium treatment that inhibits apoptosis while promoting proliferation
2Reliability
If pulse wave treated water is used to culture stem cells, then apoptosis is inhibited and repair is promoted, but the pulse treatment parameters must be precisely controlled
Solution Approach 1:
The patent employs periodic action through structured pulse sequences consisting of repeated cycles with specific duty ratios and intervals, creating a rhythmic treatment pattern that effectively inhibits stem cell apoptosis while maintaining controllable complexity through standardized cycle repetitions
3Productivity
If the pulse wave duty ratio and intervals are optimized, then stem cell proliferation is maintained and apoptosis is inhibited, but the treatment parameters become more specific and complex
Solution Approach 1:
The patent applies dynamics by allowing adjustment of pulse wave parameters including duty ratio ranges (1.3-3.5 μs), intervals (0.7-3.1 μs), power levels (28W or 35W), and frequencies (1.5M or 1.9M) to dynamically optimize stem cell culture conditions while maintaining standardized pulse sequence structures for controllable implementation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The treated water effectively inhibits apoptosis and supports stem cell proliferation without affecting normal growth, demonstrating a repair effect on stem cells, particularly human umbilical cord mesenchymal stem cells.
Implementation Method 1
adopting a pulse wave with a power of 28 W and a frequency of 1.5M to radiate and treat drinking water
Data Source
AI summary
The present invention falls within the technical field of cell repair, and specifically relates to a method for repairing stem cells and a use thereof. On the basis of the original invention technology, following improvement, in the present invention, pulse wave-treated water having the function of repairing stem cells is obtained by means of pulse wave-treated water having pulse wave sequences with equal widths and unequal intervals, and the method has additive effects on multiple treated samples. Without influencing cell proliferation, a culture system prepared from the pulse wave-treated water can not only reduce the natural apoptosis effect of human umbilical cord mesenchymal stem cells (H-MSC), but also can inhibit injury-induced H-MSC apoptosis, and has a cell repair effect.