Punch-and-Piston Biological Sample Transfer for Reduced Tissue Damage
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for transferring biological specimens, such as tissue sections, often cause damage due to strong contact forces during the transfer process, making it difficult to move samples from one surface to another without compromising their integrity.
Innovation Solution
A system comprising a punch device with a sharp bottom edge and a receiving array with pistons is used to cut and expel sample portions onto a substrate, minimizing damage by allowing controlled transfer of biological samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional transfer methods are used to move tissue sections between surfaces, then the transfer process can be completed, but strong contact forces cause damage to the tissue sample
Solution Approach 1:
The patent introduces an intermediary transfer mechanism where the tissue section is first attached to a donor substrate, then transferred to a receiving substrate through a controlled interface. The receiving substrate includes a receiving chamber that accommodates the punch device, creating a protected transfer path that minimizes direct contact forces between the tissue and external environment, thereby reducing damage during transfer.
2Ease of operation
If strong contact forces are applied during transfer, then the tissue section can be moved from one surface to another, but the tissue sample becomes damaged
Solution Approach 1:
The transfer process is segmented into distinct stages: (1) cutting the tissue section with a punch device having a sharp bottom edge, (2) transferring the cut section to a receiving substrate within a receiving chamber, and (3) securing the tissue in the final position. This segmentation allows each stage to be optimized independently, using minimal force at each step rather than applying strong forces in a single transfer action.
Solution Approach 2:
The system employs dynamic control of the transfer process where the punch device is inserted into the receiving chamber, the tissue is cut and transferred in a controlled sequence, and the receiving substrate can be adjusted to accommodate the transfer. This dynamic, multi-step approach replaces static strong-force transfer with adaptive, controlled movements that preserve tissue integrity.
3Productivity
If traditional sectioning and transfer methods are used, then tissue samples can be prepared for analysis, but the process is time-consuming and costly
Solution Approach 1:
The patent combines multiple functions into an integrated system: the punch device simultaneously cuts the tissue section and facilitates its transfer to the receiving substrate in a single operational sequence. The receiving chamber is designed to accommodate the punch device, allowing the cutting and transfer operations to be merged into one coordinated action, thereby reducing processing time and increasing productivity.
Data Source
AI summary
Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.