Pyrokinin Neuropeptide Analogues for Pollinator-Safe Insect Control

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Solution Overview

Problem

The need for greener, target-specific insecticides that minimize harm to pollinators while effectively controlling pests like aphids, moths, and fruit flies is not adequately addressed by existing broad-spectrum insecticides.

Innovation Solution

Development of natural pyrokinin neuropeptides and their analogues with insecticidal activity against hemipteran, dipteran, lepidopteran, blattodean, and coleopteran insects, while having minimal effect on important pollinators such as bees.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If broad-spectrum insecticides are used to control pest insects, then insect mortality increases, but harm to pollinators and non-target species increases

Engineering Contradiction:
Improveinsect mortalityVSAvoidharm to pollinators
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies local quality by designing neuropeptide compounds with specific sequence variations (e.g., pyroglutamate at N-terminus, specific C-terminal residues) that confer selectivity for pest insect receptors while sparing pollinators. The compound structure is locally optimized with specific amino acid substitutions to achieve differential binding affinity between target and non-target species.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent employs parameter changes by modifying key parameters of the neuropeptide structure including N-terminal pyroglutamate formation, specific residue substitutions (e.g., position 5, 8, 11), and C-terminal amide/amine variations to tune selectivity and activity. These parameter modifications enable the compound to maintain high efficacy against pests while reducing toxicity to beneficial insects.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If modified synthetic analogues of insect neuropeptides are developed for targeted insecticide use, then target-specificity improves, but regulatory approval difficulty increases

Engineering Contradiction:
Improvetarget-specificityVSAvoidregulatory approval
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent adopts a risk-averse approach by selecting conservative amino acid substitutions and maintaining high sequence homology to naturally occurring insect neuropeptides. This strategy produces compounds that are chemically simpler and more familiar to regulatory authorities, facilitating approval processes despite the goal of achieving target-specificity.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

3Productivity

If neuropeptide compounds with high insecticidal activity are used, then pest control efficacy improves, but environmental regulation complexity increases

Engineering Contradiction:
Improvepest control efficacyVSAvoidregulation complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent exploits the fact that neuropeptides are endogenous to insects, allowing them to self-target through species-specific receptor binding. This biological self-service mechanism provides inherent selectivity that simplifies regulatory assessment, as the compounds naturally differentiate between target pests and non-target species without requiring complex delivery systems or adjuvants.

Inventive Principle:
Principle #25Self-service

Data Source

PatentEP4545548B1Insect neuropeptides
Publication Date: 2025.11.19 SOLASTA BIO LTD
  • EP4545548B1 patent drawingFigure 1
  • EP4545548B1 patent drawingFigure 2
  • EP4545548B1 patent drawingFigure 3

AI summary

The present invention relates to natural or natural-like analogues of insect pyrokinin neuropeptide [Pyr]-AIMARPQVPRL-[NH2] (SEQ ID NO:2) having activity against insects, for example hemipteran, dipteran, lepidopteran, blattodean and/or coleopteran insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.