Multiplex Quantum Dot Test Cassette for Low-Interference Allergen Testing
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Solution Overview
Problem
Current commercially available products for quantitative immunochromatographic testing of allergens face challenges in performing simultaneous multiplex testing and suffer from severe interference during multiplex testing.
Innovation Solution
A multiplex quantum dot-based quantitative test card and apparatus that includes a multiplex card housing with sample loading ports and observation windows, a test strip with quantum dot-labeled antibodies, and a nitrocellulose membrane coated with test lines and a control line, enabling simultaneous multiplex testing with reduced interference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If quantum dot-labeled antibodies are used for multiplex testing, then sensitivity and measurement precision are improved, but device complexity and manufacturing difficulty increase
Solution Approach 1:
The test card is divided into multiple independent reaction slots, each capable of performing separate quantitative tests. The nitrocellulose membrane is segmented into multiple test lines with different allergen antigens, allowing simultaneous multiplex testing while maintaining individual reaction independence, thus reducing interference between tests
Solution Approach 2:
The test card housing and nitrocellulose membrane are designed as universal platforms that can accommodate multiple types of quantum dot-labeled antibodies targeting different allergens. The same structural framework supports various antibody-antigen combinations, enabling flexible multiplex testing configurations without requiring separate test devices for each allergen
2Productivity
If multiple test lines are coated on the nitrocellulose membrane for multiplex testing, then testing efficiency and productivity are improved, but cross-reactivity and interference between tests increase
Solution Approach 1:
Each test line on the nitrocellulose membrane is coated with a specific allergen antigen that binds only to its corresponding quantum dot-labeled antibody. This localized specificity ensures that reactions occur only at the intended test line, preventing cross-reactivity while enabling simultaneous multiplex testing of multiple allergens
Solution Approach 2:
The nitrocellulose membrane acts as an intermediary substrate that physically separates different antigen-antibody reactions into distinct zones. Each test line serves as an isolated reaction compartment, allowing multiple tests to proceed simultaneously without interference while maintaining the ability to detect multiple allergens in a single sample
3Measurement precision
If quantum dot-labeled antibodies are used instead of traditional labels, then fluorescence signal intensity and sensitivity are improved, but cost and manufacturing complexity increase
Solution Approach 1:
Quantum dots are pre-labeled onto antibodies during the manufacturing process, creating ready-to-use quantum dot-labeled antibodies. This preliminary labeling eliminates the need for complex labeling procedures during test card assembly, simplifying manufacturing while maintaining high fluorescence sensitivity for quantitative detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, accurate, and efficient quantitative testing of allergen-specific IgE antibodies, suitable for diagnosing allergic diseases, with improved sensitivity and reduced cross-reactivity, suitable for both clinical and self-testing applications.
Implementation Method 1
fluorescent immunoassay technology, which utilizes fluorescently labeled antibodies or antigen molecules to quantitatively analyze test samples by detecting changes in fluorescence signal intensity following specific binding
Implementation Method 2
a test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances
Data Source
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Figure 5A~5B
AI summary
Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances. By simultaneously employing quantum dot-based quantitative testing, a plurality of test lines on a nitrocellulose membrane, and a multiplex card housing, the multiplex quantum dot-based quantitative test card provided in the present application addresses both quantitative and multiplex testing challenges, thereby enhancing testing efficiency and accuracy; furthermore, it enables rapid diagnosis for various allergic diseases caused by inhaled and ingested allergens in humans.