Quantum Dot Mycotoxin Test Strip Reader for Fluorescence Quantification
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Solution Overview
Problem
Current mycotoxin testing methods, such as biological identification and chemical analysis, are either non-specific and insensitive or require complex equipment, making them unsuitable for rapid, on-site detection of mixed mycotoxin pollution in food products, which poses a significant threat to food safety and security.
Innovation Solution
An intelligent point-of-care testing device utilizing a quantum dots test strip with a shell, light sources, a light filtering component, a clamping mechanism, and a processor that captures and analyzes fluorescent signals to quantify mycotoxin content, allowing for rapid and portable detection of multiple toxins.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If traditional biological identification method is used, then the testing process is simple, but the sensitivity and specificity are poor and quantification cannot be achieved
Solution Approach 1:
The patent combines quantum dot fluorescence technology with immunochromatography test strip technology to create a hybrid detection system. The quantum dots serve as fluorescent labels that provide high sensitivity and specificity while the test strip format maintains operational simplicity, achieving both goals simultaneously
Solution Approach 2:
The patent introduces fluorescence intensity as a new measurement parameter beyond simple visual detection. By measuring the intensity of fluorescence signals from quantum dots, the system achieves quantitative analysis and significantly improved detection sensitivity while maintaining the simplicity of the test strip format
2Measurement precision
If large-scale instrument analysis (HPLC, LC-MS/MS) is used, then the detection sensitivity and accuracy are high, but the device complexity and portability are poor
Solution Approach 1:
The patent replaces complex mechanical instrumentation (HPLC pumps, LC-MS/MS systems) with a simple optical detection system using quantum dot fluorescence and a test strip reader. This substitution maintains high detection accuracy through fluorescence intensity measurement while dramatically reducing device complexity and enabling portability
Solution Approach 2:
The patent uses quantum dots as fluorescent copies or analogs of traditional enzyme labels. These quantum dot labels replicate the functionality of enzyme-linked detection methods but provide superior photostability and brightness, enabling accurate detection without requiring complex instrumentation
3Ease of operation
If traditional chemical analysis method is used, then the operation is simple and cost is low, but the use of solvents creates safety risks and the method has been phased out
Solution Approach 1:
The patent changes the detection parameter from chemical signal (color change visible to naked eye) to optical signal (fluorescence intensity measurable by device). This parameter change eliminates the need for hazardous solvents while maintaining operational simplicity and enabling quantitative analysis
Solution Approach 2:
The patent substitutes chemical analysis methods with optical detection methods. Instead of using chemical solvents and reagents for extraction and analysis, the system uses quantum dot fluorescence signals that can be detected optically, eliminating solvent safety risks while maintaining ease of operation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution enables rapid, quantitative, and portable mycotoxin testing, improving testing speed and portability while overcoming the limitations of traditional methods by providing accurate fluorescence quantification and instant results for mixed mycotoxin pollution.
Implementation Method 1
quantum dots test strip with a shell, light sources, a light filtering component, a clamping mechanism, and a processor that captures and analyzes fluorescent signals
Data Source
AI summary
The present disclosure discloses an intelligent point-of-care testing device for mycotoxins based on a quantum dots test strip. A first light source and a second light source are used for exciting a test strip which is placed in a shell and coated with a mycotoxin antigen to obtain emitted light, and a light filtering component is used for filtering out impurity light. A clamping mechanism is used for clamping and fixing an image capturing component. The image capturing component is used for capturing, an image of mycotoxins based on a quantum dots test strip. A processor is used for analyzing and calculating image information to obtain a mycotoxin content result. The testing time is shortened, and the portability and practicability of mycotoxin testing are improved. Quantitative instant testing of single or multi-toxin mixed pollution is achieved, and the technical problem of fluorescence quantification is solved.