Fluorescence Spectrophotometer Quantum Efficiency Distribution Measurement
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Solution Overview
Problem
Current fluorescence spectrophotometers face challenges in accurately measuring quantum efficiency distributions, particularly for solid samples, due to uneven reflection and limited known samples, which complicates the calculation of absolute quantum efficiency and requires correction for spectral sensitivity characteristics of the camera and optical systems.
Innovation Solution
A method involving a fluorescence spectrophotometer that irradiates a sample with excitation light and uses a photographing device with multiple channels to capture images, calculating absorption and fluorescence luminance values to determine quantum efficiency distributions by correcting for spectral characteristics and interreflection effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescence spectrophotometers are used to measure quantum efficiency, then measurement can be performed, but measurement precision deteriorates due to uneven reflection and limited known samples
Solution Approach 1:
The patent changes the measurement parameters by using multiple wavelength channels (first and second wavelength ranges) to capture different components of light (reflected excitation light and fluorescent light). This allows separate calculation of absorption luminance and fluorescence luminance, improving measurement precision by accounting for varying reflection characteristics across different wavelengths
Solution Approach 2:
The patent introduces a reference material as an intermediary to establish a relationship between captured luminance values and quantum efficiency. By measuring the reference material with known properties, the system creates a calibration basis that improves reliability when measuring unknown samples
2Measurement precision
If absolute quantum efficiency measurement is performed, then measurement accuracy improves, but device complexity increases due to correction requirements
Solution Approach 1:
The patent replaces complex mechanical correction systems with a computational approach. Instead of using additional optical components or mechanical adjustment mechanisms to correct for reflection and spectral sensitivity, the system uses image processing and calculation algorithms to compensate for these effects, thereby achieving absolute quantum efficiency measurement without increasing device complexity
Solution Approach 2:
The photographing device is designed to perform multiple functions: capturing reflected excitation light, capturing fluorescent light, and providing data for calculating both absorption and fluorescence luminance. This multi-functionality allows the same device to achieve absolute quantum efficiency measurement without requiring separate correction instruments
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables detailed analysis of quantum efficiency distributions across sample surfaces, improving accuracy and overcoming limitations of previous methods by providing a more precise and absolute measurement of quantum efficiency.
Implementation Method 1
irradiate a sample with excitation light to measure fluorescent light emitted from the sample
Data Source
AI summary
A method of obtaining a quantum efficiency distribution in a predetermined sample surface, including: irradiating a reference material with excitation light belonging to a first wavelength range; obtaining the reference material's image, which includes a first channel for the first wavelength range and a second channel for a second wavelength range, the first and the second channel's irradiation luminance value in each pixel; irradiating the predetermined sample surface with the excitation light; obtaining the first and the second channel's measurement luminance value in each pixel of the image of the predetermined surface; calculating an absorption luminance value from a difference between the first channel's irradiation luminance value and measurement luminance value; calculating a fluorescence luminance value from difference between the second channel's irradiation luminance value and measurement luminance value; calculating quantum efficiency of each pixel based on the values; and obtaining quantum efficiency distribution.