Novel Quencher Compound for Nucleic Acid Detection Stability
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Solution Overview
Problem
Current quenchers used in biotechnology for fluorescent dyes have limitations in maintaining long-term reaction and storage stability, especially in water-soluble conditions, and have limited reactive groups for binding with biomolecules, restricting their applications in optical imaging and nucleic acid detection.
Innovation Solution
A novel quencher compound represented by Chemical Formula 1, which includes various functional groups and reactive moieties, is developed for enhanced binding capabilities and stability, allowing for improved quenching efficiency and compatibility with biomolecules, and is integrated into oligonucleotides, compositions, and supports for nucleic acid detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional quenchers are used for fluorescent dyes, then quenching function is achieved, but long-term reaction and storage stability is poor in water-soluble conditions
Solution Approach 1:
The patent modifies the chemical structure of the quencher by introducing a water-soluble group (such as sulfonate or carboxylate) to change its solubility parameters. This structural modification enables the quencher to maintain stability in water-soluble conditions while preserving its quenching function, directly resolving the contradiction between stability and water-solubility.
Solution Approach 2:
The patent creates a composite quencher structure that combines the quenching moiety (such as BQ or DABCYL) with water-soluble functional groups. This composite design integrates the quenching capability with enhanced water solubility and long-term stability, allowing the quencher to function effectively in aqueous environments for extended periods.
2Adaptability or versatility
If limited reactive groups are used in quenchers, then binding to specific biomolecules is achieved, but adaptability and versatility for different applications is restricted
Solution Approach 1:
The patent designs the quencher with multiple types of reactive groups (amine-reactive, thiol-reactive, and hydroxyl-reactive groups) incorporated into a single molecular structure. This multi-functional design enables the quencher to bind to various biomolecules including proteins, peptides, and nucleic acids, significantly enhancing its adaptability and versatility across different applications without requiring multiple separate quencher molecules.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel quencher exhibits superior quenching efficiency and stability, enabling effective detection of nucleic acids through enhanced fluorescence quenching and binding properties, facilitating advanced optical imaging and nucleic acid analysis.
Implementation Method 1
Mechanisms of quenching phenomenon are known to occur through aggregation of dyes such as fluorescence resonance energy transfer (FRET), photo-induced electron transfer, and H-dimer formation.
Implementation Method 2
Mechanisms of quenching phenomenon are known to occur through aggregation of dyes such as fluorescence resonance energy transfer (FRET), photo-induced electron transfer, and H-dimer formation.
Data Source
AI summary
The present disclosure relates to a quencher having a quenching effect on a fluorescent material exhibiting luminescence characteristics at an excited energy level, and various uses thereof.