Quercetin Sugar Preserving Agent Low-Temperature Organ Protection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing preservation techniques for organs and tissues are inadequate at low temperatures, causing damage and limiting the preservation period, especially since they do not effectively combine quercetin with specific sugars.
Innovation Solution
A preserving agent and solution comprising quercetin combined with fructose or sucrose, used in a preservation method that immerses organs or tissues in a liquid mixture at low temperatures to protect against low-temperature damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If intracellular-type preserving solutions (low sodium, high potassium) are used, then the preservation period is extended, but cytotoxicity occurs when preservation temperature is increased
Solution Approach 1:
The patent modifies the chemical composition parameters of the preserving solution by introducing quercetin (a flavonoid) and specific sugars (fructose, sucrose, trehalose) in addition to the traditional intracellular-type electrolyte composition. This parameter change allows the solution to maintain low sodium and high potassium concentrations for extended preservation while adding protective components that prevent cytotoxicity at elevated temperatures through antioxidant and stabilizing effects.
Solution Approach 2:
The patent creates a composite preserving solution by combining multiple components: the intracellular-type electrolyte composition (sodium pump inhibitor), quercetin (flavonoid antioxidant), and specific sugars (fructose, sucrose, trehalose). This composite formulation synergistically provides both extended preservation capability and protection against temperature-induced cytotoxicity, resolving the contradiction between preservation duration and cellular safety.
2Duration of action of stationary object
If low temperature preservation is applied, then organ and tissue preservation is achieved, but cell damage and rupture occur
Solution Approach 1:
The patent applies beforehand cushioning by incorporating quercetin and specific sugars into the preserving solution before cell damage can occur. These components act as protective agents that cushion against low-temperature stress from the outset, preventing membrane rigidity, inhibiting ice crystal formation, and maintaining cellular integrity throughout the preservation period, thereby eliminating cell rupture while enabling extended low-temperature storage.
Solution Approach 2:
The patent changes the chemical parameters of the preserving solution by adding quercetin (at concentrations of 0.01-100 μg/mL) and specific sugars (fructose, sucrose, or trehalose at 0.01-0.8 M). These parameter modifications provide thermal protection and membrane stabilization that prevent cell damage during low-temperature preservation, allowing the solution to maintain both long preservation duration and cellular safety.
3Stress or pressure
If sodium pump function is inhibited to reduce intracellular sodium, then osmotic pressure is reduced, but ion balance regulation fails
Solution Approach 1:
The patent extracts and utilizes the inhibitory effect on the sodium pump (Na+-K+ ATPase) as a key mechanism. By incorporating agents that inhibit sodium pump function, the solution reduces intracellular sodium accumulation and osmotic pressure during preservation. This extraction of the pump inhibition mechanism allows the preserving solution to actively control ion balance, preventing water influx and cell swelling while maintaining reliable ion regulation throughout the preservation period.
Data Source
AI summary
This invention discloses a preserving agent for organs or tissue comprising (A) quercetin, and (B) at least one sugar selected from the group consisting of fructose and sucrose; a preserving solution for organs or tissue comprising the preserving agent; and a preservation method for organs or tissue comprising the step of immersing an organ or tissue in a liquid mixture comprising (A) quercetin, and (B) at least one sugar selected from the group consisting of fructose and sucrose.