Quinazoline Crystalline Forms for Brain-Penetrant ErbB2 Inhibition

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Solution Overview

Problem

Current anti-ErbB2 agents, such as monoclonal antibodies, antibody drug conjugates, and tyrosine kinase inhibitors, lack central nervous system penetrability, limiting their efficacy in treating ErbB2 positive breast cancer with brain metastases.

Innovation Solution

Development of new crystalline forms of the quinazoline compound (R)-N-(4-([1,2,4]triazolo[1,5-c]pyrimidin-7-yloxy)-3-methylphenyl)-5-((3,3-difluoro-1-methylpiperidin-4-yl)oxy)-6-methoxyquinazolin-4-amine, including complexes with fumaric acid and free bases, which exhibit improved brain penetration and selectivity against wild type EGFR.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional anti-ErbB2 agents (monoclonal antibodies, ADCs, TKIs) are used, then ErbB2 inhibition is achieved, but central nervous system penetrability is insufficient

Engineering Contradiction:
ImproveErbB2 inhibition efficacyVSAvoidlack of CNS penetrability
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent modifies the chemical parameters of the TKI structure by introducing a difluoromethylpiperidin-4-yl group and optimizing the quinazoline core structure. These parameter changes in molecular structure enable the compound to cross the blood-brain barrier while maintaining ErbB2 inhibition activity, thus resolving the contradiction between efficacy and CNS penetrability

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention creates a composite molecular structure combining quinazoline core with difluoromethylpiperidine side chain. This composite structure integrates the pharmacophore for ErbB2 binding with structural features that facilitate blood-brain barrier penetration, achieving both reliability and CNS penetrability

Inventive Principle:
Principle #40Composite materials

2Reliability

If TKIs with high ErbB2 affinity are developed, then tumor inhibition is improved, but selectivity against wild type EGFR may be compromised

Engineering Contradiction:
Improvetumor inhibitionVSAvoidside effects from EGFR inhibition
Core Design Contradiction:
ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent introduces specific local structural features (difluoromethylpiperidin-4-yl group at position 5 and methoxy group at position 6 of quinazoline) that create local binding characteristics. These local quality modifications enable selective interaction with ErbB2 ATP binding site while minimizing interaction with EGFR, thus achieving high tumor inhibition with reduced side effects

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

By changing the chemical parameters of the inhibitor structure (introducing fluorine atoms, optimizing ring substitution patterns), the patent achieves differential binding affinity where ErbB2 inhibition is strengthened while EGFR inhibition is selectively reduced, improving the therapeutic index

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP4399207B1Crystalline forms of quinazoline derivatives, preparation, composition and use thereof
Publication Date: 2025.11.26 F HOFFMANN LA ROCHE & CO AG
  • EP4399207B1 patent drawingFigure 1~2
  • EP4399207B1 patent drawingFigure 3~4
  • EP4399207B1 patent drawingFigure 5~6

AI summary

It is disclosed crystalline forms of (R) -N- (4- ( [1, 2, 4] triazolo [1, 5-c]pyrimidin-7-yloxy) -3-methylphenyl) -5- ( (3, 3-difluoro-1-methylpiperidin-4-yl) oxy) -6-methoxyquinazolin-4-amine, methods for the preparation thereof, pharmaceutical compositions comprising one or more of the crystalline forms as an active ingredient, and use of the crystalline forms in the treatment of hyperproliferative diseases.