Quinolone Detection via Antibody-Antigen Binding

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Solution Overview

Problem

Current methods for detecting quinolone antibiotics in biological samples are inefficient due to the need for complex instrumentation and skilled personnel, and existing immunochemical procedures do not effectively address the requirement for rapid and reliable analysis, especially in ensuring public health safety.

Innovation Solution

Development of haptens and immunoreactive agents that attach quinolone antibiotics to immunogenic carriers through the 1-position, allowing for the production of broad-spectrum antibodies and their use in immunochemical methods for accurate detection and quantification in biological samples.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If complex instrumentation methods (GC-MS, HPLC) are used for quinolone detection, then measurement precision is improved, but device complexity and cost increase

Engineering Contradiction:

Improvedetection accuracyVSAvoidinstrumentation complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent replaces complex mechanical/instrumental analysis systems (GC-MS, HPLC) with a biological recognition system (antibody-antigen interaction) that provides comparable precision through immunological specificity rather than physical separation and detection mechanisms

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The invention uses antibodies as biological copies/recognition elements that specifically bind to quinolone structures, enabling detection without requiring the complex physical instrumentation needed for traditional chromatographic methods

Inventive Principle:

Principle #26Copying

2Measurement precision

If complex instrumentation methods are used for quinolone detection, then measurement precision is improved, but ease of operation deteriorates

Engineering Contradiction:

Improvedetection accuracyVSAvoidoperational simplicity

Core Design Contradiction:

Measurement precisionVSEase of operation

Solution Approach 1:

The complex operational procedures of GC-MS and HPLC (sample preparation, instrument operation, data analysis) are replaced with a simpler immunological assay format that requires minimal technical skill while maintaining detection accuracy through the inherent specificity of antibody-antigen binding

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Measurement precision

If complex sample preparation is performed, then measurement precision is improved, but loss of time increases

Engineering Contradiction:

Improvedetection accuracyVSAvoidanalysis time

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The patent employs pre-formulated immunological reagents and simplified sample treatment protocols that perform necessary preparation steps in advance or with minimal intervention, eliminating the time-consuming extraction and purification steps required by chromatographic methods while preserving detection accuracy

Inventive Principle:

Principle #10Preliminary action

4Productivity

If immunochemical procedures are used for quinolone detection, then productivity is improved, but measurement precision may deteriorate

Engineering Contradiction:

Improveprocessing capabilityVSAvoiddetection accuracy

Core Design Contradiction:

ProductivityVSMeasurement precision

Solution Approach 1:

The patent optimizes immunological assay parameters (antibody concentration, incubation time, temperature, pH) to maximize both throughput and detection accuracy, demonstrating that immunological methods can achieve high productivity without sacrificing precision when properly calibrated

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention develops broad-spectrum antibodies that recognize multiple quinolone structures, enabling a single immunoassay system to detect various quinolone antibiotics simultaneously, thereby increasing productivity across different analytes while maintaining precision through consistent immunological recognition mechanisms

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The described approach enables simpler, cost-effective, and high-capacity detection of quinolone antibiotics, improving the efficiency and reliability of analysis, thereby enhancing public health protection by addressing the limitations of existing methods.

Implementation Method 1

the immunization hapten was a commercial quinolone bound to a immunogenic protein by means of carboxylic acid in the 3-position or through remaining piperazine in the 7-position

Methodology Applied

Scientific EffectCovalent bonding: Chemical Bonding

Implementation Method 2

broad spectrum antibodies against quinolones and their application in immunochemical techniques for the analysis of said antibiotics

Methodology Applied

Scientific EffectAntigen-antibody recognition:

Data Source

PatentUS10150736B2Haptens and immunoreactive agents and use thereof for producing family antibodies and immunoassays for quinolones

Publication Date: 2018.12.11 CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)

AI summary

The invention relates to haptens, immunogens and secondary immunoreactive agents, to the use thereof for producing wide-spectrum antibodies against quinolone-type antibiotics, to the application thereof to immunochemical analysis techniques, and to a kit enabling the detection of said antibiotics in biological samples from food products of animal origin.