Inbred Sweet Corn Line R373D Molecular Marker Breeding

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Solution Overview

Problem

The development of high-yielding, agronomically sound sweet corn hybrids is hindered by the unpredictability of genotype interactions with environmental conditions and the laborious process of identifying and reproducing superior inbred lines with desired traits, which requires extensive research resources.

Innovation Solution

The introduction of the inbred sweet corn line R373D, which is homozygous and stable, with specific single gene traits introgressed, allowing for controlled pollination and production of hybrid seeds with desirable characteristics such as disease resistance and uniformity, using techniques like RFLP and SSR for genetic analysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop sweet corn hybrids, then various desirable traits can be combined in a single variety, but the process is laborious and requires extensive research resources

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent utilizes molecular marker parameters (RFLP and SSR markers) to track and select for desirable traits during breeding. By changing from traditional phenotypic selection to genotypic selection using molecular markers, the breeding process becomes more efficient and less laborious while maintaining the ability to combine multiple desirable traits in a single variety

Inventive Principle:
Principle #35Parameter changes

2Stability of the object's composition

If extensive research resources are allocated to identify and reproduce superior inbred lines, then uniformity and stability of hybrids can be ensured, but the time and cost increase significantly

Engineering Contradiction:
Improveuniformity and stability of hybridsVSAvoidtime for identifying and reproducing superior inbreds
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent employs molecular marker analysis (RFLP and SSR) as a preliminary action to identify superior inbred lines before actual hybrid production. This allows researchers to screen and select promising inbreds early in the breeding process based on their genetic makeup, rather than waiting for field performance data, thereby reducing the time required to identify and reproduce superior lines while ensuring uniformity and stability

Inventive Principle:
Principle #10Preliminary action

3Measurement precision

If molecular marker techniques like RFLP and SSR are used for genetic analysis, then genetic identity and desirable traits can be maintained, but the manufacturing precision requirements increase

Engineering Contradiction:
Improvegenetic analysis accuracyVSAvoidbreeding process precision
Core Design Contradiction:
Measurement precisionVSManufacturing precision

Solution Approach 1:

The patent uses molecular markers (RFLP and SSR) as genetic copies or fingerprints to verify and maintain the identity of superior inbred lines. These markers serve as reliable genetic signatures that can be copied and compared across different breeding generations, ensuring that the desired genetic composition is maintained without requiring extremely high manufacturing precision in the breeding process itself

Inventive Principle:
Principle #26Copying

Data Source

PatentUS7816588B2Inbred sweet corn line R373D
Publication Date: 2010.10.19 SYNGENTA CROP PROTECITON AG

AI summary

An inbred sweet corn line, designated R373D, the plants and seeds of inbred sweet corn line R373D, methods for producing a maize plant produced by crossing the inbred sweet corn line R373D with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred sweet corn line R373D with another maize line or plant.