Raman Analyte Detection with Fluorescence-Quenching Samples
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Solution Overview
Problem
The strong fluorescence effect in organic matrices limits the detection of analytes using Raman spectroscopy, making it difficult to obtain clear Raman spectra.
Innovation Solution
A method involving a fluorophore, analyte, and quencher is used to form a sample, where the quencher quenches the fluorophore's fluorescence, allowing for Raman spectroscopy to determine the analyte.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If Raman spectroscopy is performed on samples in organic or aqueous matrices, then qualitative and quantitative information of analytes can be obtained, but strong fluorescence effects from the sample and matrix components limit the detection capability
Solution Approach 1:
The patent introduces a quencher as an intermediary substance that selectively interacts with fluorescent components in the sample matrix. The quencher molecules bind to fluorescent substances and transfer their energy, effectively quenching the fluorescence signal without interfering with the Raman scattering process. This mediator approach allows the Raman spectroscopy to proceed with minimal fluorescence interference, resolving the contradiction between obtaining analytical information and avoiding fluorescence effects
Solution Approach 2:
The patent utilizes the principle of parameter changes by modifying the optical properties of the sample system through the addition of quenchers. By changing the concentration and type of quenchers, the fluorescence lifetime and intensity parameters are altered, allowing the Raman signal to emerge from the fluorescent background. This parameter modification enables effective analyte detection in matrices that would otherwise produce strong fluorescence interference
2Object-affected harmful factors
If fluorescence quenching is performed by reducing fluorescence lifetime using KI, then some fluorescence suppression is achieved, but molecular quenching has never been successfully performed
Solution Approach 1:
The patent applies the principle of converting harm into benefit by transforming the harmful fluorescence effect into a useful analytical tool. Instead of merely suppressing fluorescence through simple lifetime reduction, the invention uses molecular quenching mechanisms where the quencher-fluorophore interactions create specific spectral signatures. The fluorescence quenching process itself becomes a beneficial mechanism for enhancing Raman signal detection, as the selective quenching allows differentiation between fluorescent and Raman signals, improving overall analytical reliability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively reduces fluorescence interference, enabling reliable and fast Raman spectroscopy even in systems with significant fluorescence, enhancing the detection of analytes.
Implementation Method 1
a fluorophore, which is capable of emitting fluorescent electromagnetic radiation when exciting with the monochromatic electromagnetic radiation having the maximum excitation wavelength λmax1
Implementation Method 2
Providing a quencher, which is capable of quenching the fluorophore's fluorescent electromagnetic radiation
Implementation Method 3
Raman spectroscopy is a technique specialized in measuring the frequency shift of inelastic scattered light from the sample when the photon from incident light strikes a molecule and produce a scattered photon
Data Source
AI summary
The present invention relates to a method for determining at least one analyte of interest and the uses thereof. The present invention further relates to a diagnostic system, a kit and the use thereof for determining at least one analyte of interest.