Integrated Raman Spectroscopy and Light Microscopy System
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Solution Overview
Problem
Current multi-modality spectro-microscopic systems fail to simultaneously perform in-situ microscopic and spectroscopic measurements on the same sample, especially for samples undergoing continuous transformations, limiting the understanding and optimization of mesoscale materials and their functional behaviors.
Innovation Solution
A novel high-sensitivity multi-functional spectro-microscopic system integrating far-field optical microscopy imaging with Raman-based spectroscopic techniques, including confocal Raman and surface-enhanced Raman spectroscopy, for simultaneous acquisition of morphological and chemical information on nanoscale and mesoscale structures, using a reflected differential interference contrast microscope optically coupled with a Raman spectroscope and total internal reflection fluorescence/scattering microscope.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple microscopy modes are used for sample positioning and fluorescence image acquisition, then basic imaging functions are achieved, but advanced features such as single molecule sensitivity, sub-diffraction-limited spatial resolution, and fast temporal resolution cannot be combined with spectroscopic measurements
Solution Approach 1:
The patent merges advanced microscopy modes (single molecule fluorescence imaging, super-resolution imaging, fast temporal resolution imaging) with Raman spectroscopy in a single integrated system. The optical path is designed to allow simultaneous acquisition of both imaging and spectroscopic data from the same sample region, enabling correlation between morphological and chemical information.
Solution Approach 2:
The system is designed as a universal platform that can perform multiple functions: fluorescence imaging, dark-field imaging, Raman spectroscopy, and spectroscopic imaging. A single optical system accommodates various imaging modes and spectroscopic techniques, making the system adaptable to different research requirements without requiring separate instruments.
2Loss of information
If spectroscopic techniques are used as companion tools to gain structural fingerprints, then chemical information is obtained, but physical and chemical characteristics cannot be provided simultaneously
Solution Approach 1:
The patent combines Raman spectroscopy with advanced light microscopy in a single integrated system where the optical paths are designed to simultaneously acquire both imaging and spectroscopic data. This merging eliminates the need for separate companion tools and enables simultaneous acquisition of physical and chemical characteristics from the same sample region.
3Measurement precision
If in-situ microscopic and spectroscopic measurements are performed on the same sample, then comprehensive characterization is achieved, but the system complexity and difficulty of simultaneous measurement increase
Solution Approach 1:
The optical system is segmented into distinct but integrated pathways: one for fluorescence imaging, one for Raman spectroscopy, and one for dark-field imaging. Each pathway can be independently optimized and controlled, allowing simultaneous measurements without excessive complexity. The segmentation enables independent adjustment of imaging and spectroscopic parameters while maintaining synchronization.
Solution Approach 2:
The patent introduces an intermediary optical system that mediates between the light source, sample, and detectors for both imaging and spectroscopy. This intermediary architecture allows coordinated control of multiple measurement processes, enabling simultaneous acquisition of imaging and spectroscopic data through a unified optical management system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables dynamic in-situ acquisition of chemical and morphological information with sub-micron spatial resolution, correlating physical and chemical changes in samples under various environmental conditions, enhancing the characterization and understanding of mesoscale materials.
Implementation Method 1
a Raman spectroscope optically coupled with the RDIC microscope such that the integrated spectro-microscopic system is capable of simultaneously acquiring both RDIC images and Raman spectra on the same sample
Implementation Method 2
reflected differential interference contrast (RDIC) microscope
Implementation Method 3
total internal reflection fluorescence/scattering (TIRF/TIRS) microscope
Data Source
AI summary
An integrated spectro-microscopic system for multimodality imaging on a sample includes a reflected differential interference contrast (RDIC) microscope, a Raman spectroscope optically coupled with the RDIC microscope and a total internal reflection fluorescence/scattering (TIRF/TIRS) microscope optically coupled with the RDIC microscope such that the integrated spectro-microscopic system is capable of simultaneously acquiring both the RDIC images, the Raman spectra and TIRF/TIRS images on the same sample.