Rape BGR Gene for Compact Architecture and Yield
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Solution Overview
Problem
Current oilseed rape varieties face challenges such as high plant height, wide canopy, and easy lodging, leading to low average yield per unit area. The 'Green Revolution' gene has not been applied in oilseed rape breeding, hindering the development of ideal plant architecture.
Innovation Solution
The oilseed rape green revolution gene bgr, derived from a mutant of Brassica rapa, encodes a protein with a mutation in the glycogen synthase kinase 3β (GSK3β) protein, specifically changing E to K in the conserved motif LGTPTRE. This gene is used to introduce mutations into the oilseed rape genome, reducing plant height, compacting plant architecture, and enhancing yield and oil content.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional oilseed rape varieties are used, then plant height is maintained at normal levels, but plant architecture becomes wide and lodging resistance decreases
Solution Approach 1:
The patent applies parameter changes by introducing a specific point mutation (E293K) in the GSK3β protein sequence, which alters the biochemical parameters of the plant to achieve semi-dwarfing phenotype. This single amino acid substitution changes the enzyme activity and subsequently modifies plant height and architecture parameters, resolving the contradiction between maintaining normal height and improving lodging resistance.
Solution Approach 2:
The invention applies local quality by targeting a specific locus (BnaA04g022200) in the oilseed rape genome for mutation. By modifying only this specific gene rather than the entire genome, the patent achieves localized changes in plant architecture (reduced height, compact canopy) while preserving other important traits, thus improving lodging resistance without completely altering plant height to detrimental levels.
2Productivity
If plant height is reduced to improve lodging resistance, then harvest index increases, but yield per unit area may be affected
Solution Approach 1:
The patent uses parameter changes to optimize the relationship between plant height and yield. By precisely controlling the E293K mutation in GSK3β, the invention achieves an optimal plant height range (120-140 cm) that balances lodging resistance with productive capacity. This specific parameter modification ensures that harvest index increases while maintaining sufficient biomass accumulation for high yield per unit area.
Solution Approach 2:
The invention applies dynamics by creating a semi-dwarfing effect rather than complete dwarfism, allowing the plant to maintain dynamic adaptability in different growing conditions. The modified GSK3β protein provides a balanced response that adjusts plant architecture dynamically, ensuring both lodging resistance and adequate yield potential across varying environmental conditions.
3Shape
If the bgr gene is introduced to create ideal plant architecture, then branching angle is reduced and plant architecture is compacted, but breeding complexity increases
Solution Approach 1:
The patent applies parameter changes by focusing on a single critical parameter (amino acid position 293 in GSK3β) that controls plant architecture. By mutating only this specific position rather than multiple genes, the invention achieves compact plant architecture with reduced branching angle while minimizing breeding complexity. This single-point mutation approach simplifies the breeding process compared to traditional multi-gene manipulation.
Solution Approach 2:
The invention applies the extraction principle by isolating and modifying only the essential functional element (the E293K mutation in BnaA04g022200) responsible for plant architecture control. By extracting this specific genetic element from the complex genome and applying it separately through gene editing or transgenic approaches, the patent simplifies the breeding process while achieving the desired compact plant architecture and reduced branching angle.
Data Source
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AI summary
Provided are a rape green revolution gene bgr and a use thereof. The gene bgr can significantly reduce the height of rape plants, achieve thick and strong stems, lodging resistance, a reduced branching angle, and a compact plant type, and can effectively increase the harvest index. The gene has remarkable heterosis, heterozygous bgr can enable tetraploid rape (Brassica napus and Brassica juncea) to have more ideal plant type characteristics, the plant height is 120-160 cm, the plant type is compact, the branching angle is small (less than 30°), the first branching position is lowered, the harvest index is increased by about 29.03%, the gene is suitable for close planting, and the yield is greatly increased by 11-27%. The oil content of hybrid Brassica napus having bgr is greatly increased and can reach 51.7-54.86%, and compared with the oil contents of common high-oil-content rape varieties, the oil content is increased by 9.14-19.26%. A valuable genetic resource is provided for rape breeding, and an effective means is provided for creation of new rape germplasm.