Rapid Viral Detection System Using Mobilizable Antibody Conjugates
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Solution Overview
Problem
There is a need for rapid and accurate diagnostic tests that can detect SARS-CoV-2 and differentiate between coronavirus and influenza viruses at the point-of-care with minimal exposure to infectious agents, allowing for timely therapeutic intervention.
Innovation Solution
A rapid detection system comprising a sample pad, a conjugate pad, and a membrane with immobilized antibodies that form mobilizable and immobilizable complexes to detect SARS-CoV-2 antigens, capable of producing visible signals without instrumentation, using a phosphate buffered saline solution and specific antibodies like Antibody B and Antibody A for SARS-CoV-2, and additional antibodies for influenza viruses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If rapid detection methods are used, then detection time is reduced, but detection precision may be compromised
Solution Approach 1:
The detection system is divided into distinct functional zones: a sample application zone, a conjugate pad zone containing mobilizable antibody-conjugate complexes, a membrane zone with immobilized capture antibodies, and a detection zone. This segmentation allows each component to perform its function optimally within a rapid timeframe while maintaining overall detection accuracy through coordinated action of all zones.
Solution Approach 2:
Antibody-conjugate complexes are pre-formed and mobilizable within the conjugate pad before sample application. This preliminary preparation of detection reagents ensures that when the sample is applied, the detection reaction can proceed immediately without delays associated with reagent preparation, thus reducing total detection time while preserving accuracy through pre-optimized antibody-conjugate interactions.
2Adaptability or versatility
If multiple viruses are detected simultaneously, then diagnostic versatility is improved, but device complexity increases
Solution Approach 1:
The test system incorporates multiple immobilized capture antibodies on the membrane, each specific to different viral antigens (e.g., SARS-CoV-2 nucleocapsid protein, influenza A, influenza B). The single conjugate pad contains mobilizable antibody-conjugate complexes that can bind to multiple different viral antigens. This universal design allows one test device to detect multiple different viruses simultaneously, achieving diagnostic versatility without requiring separate tests for each virus, thus managing complexity efficiently.
3Ease of operation
If point-of-care testing is implemented, then accessibility is improved, but exposure risk to infectious agents increases
Solution Approach 1:
The system uses immobilized capture antibodies on the membrane as intermediaries that capture viral antigens from the patient sample. The mobilizable antibody-conjugate complexes in the conjugate pad serve as additional intermediaries that bind to captured antigens and transport them to detection zones. These intermediary antibody complexes mediate the detection process, allowing the test to be performed at point-of-care with minimal direct handling of infectious material, as the antibodies act as protective barriers that bind and contain the viral antigens throughout the testing process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, accurate, and efficient detection of SARS-CoV-2 and influenza viruses in a single test, reducing exposure to infectious agents and facilitating timely diagnosis and treatment.
Implementation Method 1
The first anti-SARS-CoV-2 antibody of the mobilizable conjugate is capable of binding to a SARS-CoV-2 antigen to form a mobilizable conjugate-antigen complex
Implementation Method 2
The second anti-SARS-CoV-2 antibody is capable of binding to the mobilizable conjugate-antigen complex to form a first immobilizable complex
Implementation Method 3
The immunoglobulin is capable of binding to the mobilizable conjugate to form a second immobilizable complex
Implementation Method 4
a sample pad comprising a porous material through which the sample can flow by capillary action
Data Source
AI summary
The disclosure provides compositions and methods for detecting coronaviruses, in particular SARS-CoV-2, in biological samples. The disclosure also provides compositions and methods for simultaneously detecting coronaviruses and influenzaviruses in biological samples.