Rare Cell Filtration for Bias-Free Multi-Analysis
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Solution Overview
Problem
Existing methods for isolating rare cells from biological samples suffer from issues of cell loss, selection bias, and incompatibility with subsequent analytic procedures, making them unsuitable for reliable diagnostic and therapeutic applications.
Innovation Solution
The method employs filtration using filters with specific pore sizes and densities to isolate rare cells, allowing for their subsequent analysis and characterization through cytomorphology, immunolabeling, and molecular analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If filtration is used to isolate rare cells, then cell loss and selection bias are eliminated, but the complexity of the isolation process increases
Solution Approach 1:
The patent employs filtration membranes with specific pore sizes (e.g., 3-10 μm) to physically separate rare cells from biological samples. The porous structure allows smaller cells and debris to pass through while retaining larger rare cells, achieving reliable isolation without requiring complex biological markers or multi-step procedures
Solution Approach 2:
The filtration process directly extracts rare cells from complex biological matrices by passing samples through filters, isolating the target cells in a single step without needing complex sequential separation techniques or multiple purification stages
2Measurement precision
If multiple analytic procedures are performed on isolated rare cells, then diagnostic accuracy improves, but the time and resource consumption increases
Solution Approach 1:
The isolated rare cells are preserved in a state that allows multiple different analytical procedures (cytomorphology, immunolabeling, molecular analysis) to be performed on the same cell population, enabling comprehensive diagnostic evaluation without requiring separate cell isolations for each test
Solution Approach 2:
The filtration step performs preliminary isolation and concentration of rare cells before any analytical procedures, preparing the sample in advance for multiple downstream applications and reducing the time needed for each subsequent analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively isolates and characterizes rare cells without loss or bias, enabling multiple analytic procedures and facilitating personalized medicine and non-invasive diagnostics.
Implementation Method 1
isolating or extracting rare cells by passing a biological sample through a filter and recovering the isolated rare cells on the filter; wherein the filter has a pore size, pore density or other physical characteristics that retain rare cells but which permit passage of other kinds of cells
Data Source
AI summary
A process for isolating or extracting rare cells from a biological sample including filtering a biological sample, which may be treated or diluted, through a filter that has a pore size, pore density or other physical properties that retain rare cells, but which permits other kinds of cells to pass through the filter.