CRISPR Editing RAY1 Gene for Rice Yield and Blast Resistance
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Solution Overview
Problem
Current methods fail to effectively improve rice yield and rice blast resistance, with rice blast causing significant yield loss and quality deterioration.
Innovation Solution
Inhibiting the activity of the RAY1 protein in rice varieties using CRISPR/Cas9 technology to increase yield, grain size, plant height, and blast resistance by introducing targeted mutations or deletions in the RAY1 gene, thereby enhancing panicle development and resistance traits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional breeding methods are used to improve rice yield, then yield may be slightly improved, but rice blast resistance deteriorates and yield loss reaches 11%-30%
Solution Approach 1:
The patent extracts and removes the harmful RAY1 protein function from rice plants through CRISPR/Cas9-mediated gene editing. By targeting and disabling the RAY1 gene, the invention eliminates the negative effect of RAY1 on rice blast resistance while preserving its role in panicle development, thereby resolving the contradiction between yield improvement and disease resistance
Solution Approach 2:
The patent converts the harmful effect of RAY1 protein (which promotes blast susceptibility) into a benefit by selectively disabling its disease-promoting function while maintaining its panicle development function. The CRISPR/Cas9 system achieves this by introducing specific mutations that abolish RAY1's pathogenicity-enhancing role without completely eliminating its beneficial developmental functions
2Productivity
If rice panicle type is optimized for high yield, then yield increases, but the complexity of gene regulation increases
Solution Approach 1:
The patent simplifies the complex gene regulation network by extracting and removing the RAY1 gene's negative regulatory influence. By disabling a single key gene (RAY1) that acts as a negative regulator, the invention achieves simplified control over panicle development and disease resistance traits without needing to manipulate multiple complex regulatory pathways
Solution Approach 2:
The patent changes the functional state of the RAY1 gene from active to inactive through CRISPR/Cas9-mediated mutations. This parameter change (from functional to non-functional RAY1) simultaneously optimizes multiple traits including panicle structure, grain yield, and blast resistance, thereby simplifying the overall regulatory complexity while improving productivity
Data Source
AI summary
The invention discloses a method for improving rice yield and a protein used thereof. The invention provides a method for cultivating the target rice, comprising the following steps of inhibiting the activity of RAY1 protein in original rice to obtain target rice; compared with the original rice, the target rice shows higher yield and/or larger grain size and/or stronger resistance to rice blast and/or higher plant height and/or longer stem internode length; the RAY1 protein is a protein composed of an amino acid sequence shown as SEQ ID No. 1 in a sequence list. The invention uses CRISPR/Cas9 technology to realize site-directed editing rice RAY1 gene, through knocking out rice RAY1 gene by frameshift mutation, the protein RAY1 is inactivated, and a new generation of rice germplasm with significantly improved yield is obtained.