Rayleigh and Raman Scattering Imaging Without Labelling Agents
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Solution Overview
Problem
Current methods for obtaining chemical and material specific information in biological and medical imaging rely on invasive labelling or staining agents, leading to issues like photo-bleaching and photo-toxicity.
Innovation Solution
A method and apparatus utilizing Rayleigh and Raman scattering to determine chemical and material specific information based on changes in intensity of scattered light with varying wavelengths and polarization states, without the need for exogenous labelling agents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If labelling or staining agents are used to obtain chemical and material specific information, then measurement precision is improved, but object-generated harmful factors worsen due to photo-bleaching and photo-toxicity
Solution Approach 1:
The invention extracts and utilizes the intrinsic optical properties (Rayleigh and Raman scattering) of the sample itself, eliminating the need for exogenous labelling agents. By detecting the scattered light signals that naturally occur in biological samples, the method obtains chemical and material specific information without introducing external substances that cause photo-bleaching and photo-toxicity
Solution Approach 2:
The sample serves itself by providing the scattering signals needed for analysis. The biological sample's own molecular structure generates the Rayleigh and Raman scattering signals that are detected and analyzed, making the sample self-sufficient for obtaining chemical information without requiring external labelling agents
2Measurement precision
If multiple images with different wavelengths and polarization states are captured, then measurement precision is improved, but loss of time increases due to multiple measurements
Solution Approach 1:
The invention employs periodic modulation of the incident light's polarization state and wavelength, capturing images at different phases of this periodic variation. By analyzing the periodic changes in scattered light intensity corresponding to different polarization states and wavelengths, the method extracts chemical and material specific information efficiently
Solution Approach 2:
The invention captures more polarization states and wavelength information than the minimum single measurement would provide, but not all possible combinations. By selecting specific polarization angles and wavelength points that maximize the information content for chemical identification, the method achieves high measurement precision while limiting the total number of images required
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and non-invasive determination of chemical and material information, reducing phototoxicity and improving imaging accuracy by exploiting the wavelength-polarization signature of different chemicals and materials.
Implementation Method 1
The scattered light comprises an elastic scattering component that is due to Rayleigh scattering of the incident light in at least a portion of the sample
Implementation Method 2
The scattered light comprises an inelastic scattering component that is due to Raman scattering of the incident light in at least a portion of the sample
Data Source
AI summary
A method for obtaining chemical and/or material specific information of a sample based on scattered light. The method comprises receiving detection data comprising at least two images. Each image is indicative of the intensity of scattered light i) for incident light of a different wavelength, or ii) for incident light of a different polarization state, or iii) of a different polarization state. The scattered light comprises an elastic scattering component that is due to Rayleigh scattering of the incident light in at least a portion of the sample. Alternatively, each image is indicative of the intensity of scattered light i) of a different wavelength, or ii) for incident light of a different polarization state, or iii) of a different polarization state, wherein the scattered light comprises an inelastic scattering component that is due to Raman scattering of the incident light in at least a portion of the sample. The method further comprises determining the chemical and/or material specific information of the sample based on the change in intensity of the elastic scattering component in dependence on the change in wavelength and/or the change in polarization state of the incident and/or scattered light.