RCAT Oxidative Stress Models for Nrf2/SKN-1 Regulator Screening

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Solution Overview

Problem

The regulation of oxidative stress through Nrf2/SKN-1 transcription remains elusive, and there is a lack of understanding of biological regulators for this process, hindering effective oxidative stress research and drug screening.

Innovation Solution

Development of an animal model using a Caenorhabditis elegans RCAT gene deletion mutant and a human HEK293T cell line overexpressing RCAT, which increases ROS levels and enhances Nrf2/SKN-1 transcriptional activity, facilitating oxidative stress research and antioxidant drug screening.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If Nrf2/SKN-1 transcriptional regulation is studied without a specific animal model, then general oxidative stress mechanisms can be observed, but the specific biological regulators and their functions remain elusive

Engineering Contradiction:

Improveunderstanding of biological regulatorsVSAvoidcomplexity of regulatory mechanism

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent creates a copied system by introducing the RCAT gene from C. elegans into human HEK293T cells, establishing a heterologous expression system that replicates the native regulatory mechanism in a controllable model, enabling precise study of RCAT-Nrf2/SKN-1 interactions

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent uses RCAT as an intermediary molecule that mediates between oxidative stress signals and Nrf2/SKN-1 transcriptional activation, allowing researchers to study the regulatory mechanism through this specific intermediary rather than the entire complex pathway

Inventive Principle:

Principle #24Intermediary (Mediator)

2Productivity

If conventional animal models are used for oxidative stress research, then general oxidative stress responses can be observed, but effective drug screening for antioxidants is hindered due to lack of specific regulatory mechanisms

Engineering Contradiction:

Improveefficiency of antioxidant drug screeningVSAvoidreliability of oxidative stress model

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent changes key parameters of the model system by establishing specific RCAT expression levels and ROS generation rates in HEK293T cells, creating a standardized model with controllable oxidative stress parameters that enables reliable and efficient drug screening

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent creates a universal model system that can serve multiple functions: studying RCAT transcriptional regulation, observing oxidative stress responses, and screening antioxidant drugs, thereby improving productivity across multiple research applications

Inventive Principle:

Principle #6Universality (Multi-functionality)

3Measurement precision

If RCAT gene deletion mutant is created in C. elegans, then specific oxidative stress phenotypes and increased ROS generation can be observed, but the model requires sophisticated genetic manipulation

Engineering Contradiction:

Improveobservation of oxidative stress phenotypesVSAvoidease of creating genetic mutant

Core Design Contradiction:

Measurement precisionVSEase of manufacture

Solution Approach 1:

The patent extracts the RCAT gene function from the complex C. elegans genetic system and transfers it to human HEK293T cells, simplifying the model creation process while preserving the essential oxidative stress phenotypes and regulatory mechanisms

Inventive Principle:

Principle #2Taking out (Extraction)

Data Source

PatentUS12507678B2Animal model for oxidative stress research and use thereof

Publication Date: 2025.12.30 IND ACADEMIC COOP FOUND YONSEI UNIV

AI summary

The present invention relates to an animal model for oxidative stress research and use thereof, and more specifically, the present invention can utilize a mutant of RCAT having a regulatory function for an antioxidant stress regulator in Caenorhabditis elegans and a human cell line expressing RCAT as animal and human cell line models for oxidative stress research, using the mutant and the human cell line.