rcSso7d Proteins for Rapid Bacterial Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for detecting foodborne pathogens like Listeria monocytogenes, Salmonella, and E. coli are time-consuming, labor-intensive, and often require expensive equipment, with existing antibodies lacking specificity and requiring lengthy pre-enrichment steps.
Innovation Solution
Development of antigen-binding molecules, specifically reduced-charge Sso7d (rcSso7d) proteins, which are engineered to bind selectively to bacterial surface proteins such as LMOf2365_0639, OmpA, OmpW, OmpC, OmpF, and phoE, enabling rapid and specific detection without the need for complex equipment or extensive pre-enrichment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional detection methods are used for foodborne pathogens, then detection can be performed with existing technology, but the process is time-consuming and requires lengthy pre-enrichment steps
Solution Approach 1:
The patent applies preliminary action by pre-engineering the rcSso7d proteins with specific binding affinities to bacterial surface proteins during the protein development phase. This allows the detection system to be ready for immediate use without requiring time-consuming pre-enrichment steps, as the proteins are specifically designed to bind directly to target bacteria at various stages of growth
Solution Approach 2:
The patent employs parameter changes by modifying the charge characteristics of the Sso7d protein to create the reduced-charge variant (rcSso7d). This parameter modification enhances the protein's binding affinity and specificity to bacterial surface proteins, enabling rapid detection without pre-enrichment while maintaining high detection precision
2Measurement precision
If existing antibodies are used for detection, then detection can be performed, but they lack specificity and require complex equipment
Solution Approach 1:
The patent replaces expensive, complex antibodies with engineered rcSso7d proteins that are simpler, more stable, and can be produced cost-effectively. These proteins serve as disposable detection reagents that maintain high specificity while eliminating the need for complex equipment and extensive preparation procedures
Solution Approach 2:
The patent modifies the physical-chemical parameters of the detection protein by reducing the charge of Sso7d to create rcSso7d, which enhances stability and binding characteristics. This parameter change allows the protein to function effectively as a specific detector without requiring the complex structure and equipment needed for traditional antibodies
3Measurement precision
If pre-enrichment steps are performed to improve detection sensitivity, then detection sensitivity increases, but the process becomes labor-intensive and time-consuming
Solution Approach 1:
The patent performs preliminary action by engineering the rcSso7d proteins during development to have high binding affinity and sensitivity for target bacterial surface proteins. This preliminary optimization eliminates the need for labor-intensive pre-enrichment steps, as the proteins can detect bacteria directly at various concentrations and growth stages
Solution Approach 2:
The patent extracts and targets specific surface proteins from bacterial cells (such as OmpA, OmpW, and other outer membrane proteins) to design the binding specificity of rcSso7d. By focusing on these specific surface targets, the system achieves high detection sensitivity without requiring the extraction and enrichment of intracellular components
Data Source
AI summary
Described herein are antigen-binding molecules that bind to bacteria (e.g., Listeria monocytogenes) and methods of use thereof. Also described herein are compositions and kits comprising these antigen-binding molecules.