Reaction Cell Image Gradient Analysis for Assay Target Location
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Solution Overview
Problem
Existing methods for performing assays on solid or porous media face challenges due to non-flat image profiles caused by axis-symmetric gradients, imprecisions in sample dispensing, and optical defects, leading to reduced assay accuracy and signal degradation.
Innovation Solution
A method and apparatus for locating a target location on a reaction cell by obtaining images, creating derivative data, determining image gradient points, and identifying the center of a homogeneous region within the fluid sample, while removing image defects to improve assay precision and accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a pre-determined location is used for performing the assay, then the assay procedure is simple, but the accuracy is reduced due to large variations from non-flat image profiles
Solution Approach 1:
The patent performs preliminary image acquisition and gradient analysis to determine the actual center location of the fluid sample before performing the assay measurement. This preliminary action identifies the optimal measurement location based on the actual sample distribution, resolving the contradiction by preparing the correct target location in advance rather than using a fixed pre-determined location.
2Adaptability or versatility
If the sample dispensing location is not precisely controlled, then the dispensing process is more tolerant to variations, but the image profile becomes non-flat with axis-symmetric gradients reducing assay accuracy
Solution Approach 1:
The patent uses image gradient analysis to detect the actual center location of the fluid sample and provides feedback to adjust the assay measurement location. The gradient calculation identifies the peak location where the sample is actually dispensed, and this information is used to correct the measurement position, thereby maintaining assay accuracy despite variations in dispensing precision.
3Device complexity
If optical defects and unintended flow patterns are present, then the assay system remains simple, but the assay signal is degraded
Solution Approach 1:
The patent extracts and removes the effects of optical defects and unintended flow patterns from the image data through gradient analysis. By calculating the gradient of the image profile and identifying the true center location, the method separates the signal of interest from background artifacts and defects, thereby improving assay signal quality without adding significant system complexity.
Data Source
AI summary
The present disclosure is directed to a method and apparatus for locating a target location on a reaction cell and using the target location to perform an assay. In an example embodiment, a method of performing at least one assay includes obtaining at least one image of a fluid sample located on a reaction cell and creating a set of derivative data including a plurality of derivative data points based on the at least one image. The method also includes determining an image gradient data point for each of the plurality of derivative data points and determining a target location of the fluid sample in the reaction cell based on the image gradient data points. The method further includes performing at least one assay using the target location of the fluid sample in the reaction cell.