Reaction Transition Point Detection in Noisy Photometric Curves

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Solution Overview

Problem

Existing methods for determining transition points in chemical reactions, such as the start and end of exponential growth in PCR, suffer from low Signal to Noise Ratio (SNR) and noise from chemical and electronic sources, limiting accurate identification of reaction products and their magnitude.

Innovation Solution

A system and method that calculates a function with a line of best fit parallel to a linear function connecting two points in a chemical reaction, identifies transition points by calculating differences between this function and measured values, and uses these differences to pinpoint phase transitions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional photometric methods are used to detect reaction products, then real-time detection capability is achieved, but measurement precision is limited due to low Signal to Noise Ratio and noise from chemical and electronic sources

Engineering Contradiction:
Improveprecision of determining reaction product productionVSAvoidSignal to Noise Ratio
Core Design Contradiction:
Measurement precisionVSReliability

Solution Approach 1:

The patent segments the reaction curve analysis into multiple distinct phases (baseline phase, exponential growth phase, plateau phase) and identifies transition points between these phases. By dividing the continuous reaction process into discrete segments with characteristic behaviors, the method can precisely locate transition points even in noisy data, thereby improving measurement precision without requiring high signal-to-noise ratio throughout the entire reaction.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent performs preliminary actions by pre-defining phase boundaries and transition point criteria before analyzing the actual reaction data. The system prepares reference models of expected reaction phases and uses these to guide the identification process, allowing it to accurately pinpoint transition points even when the measured signal is contaminated with noise from chemical and electronic sources.

Inventive Principle:
Principle #10Preliminary action

2Measurement precision

If transition points are identified using traditional methods, then reaction phase detection is achieved, but accuracy is limited due to noise from chemical and electronic sources

Engineering Contradiction:
Improveaccuracy of transition point identificationVSAvoidnoise from chemical and electronic sources
Core Design Contradiction:
Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of noise into a beneficial feature by using the statistical properties of noise to distinguish true transition points from random fluctuations. The method identifies transition points based on systematic changes in the reaction curve that persist despite noise, effectively using the presence of noise to validate the robustness of identified transition points. This allows accurate transition point identification even in the presence of chemical and electronic noise sources.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The system performs preliminary filtering and validation of transition point candidates by comparing them against expected phase transition patterns. Before finalizing transition point identification, the method pre-processes the data to distinguish systematic phase transitions from random noise, thereby improving accuracy despite the presence of harmful noise from chemical and electronic sources.

Inventive Principle:
Principle #10Preliminary action

3Productivity

If real-time monitoring of chemical reactions is implemented, then reaction control capability is improved, but system complexity increases due to the need for continuous data acquisition and analysis

Engineering Contradiction:
Improvereal-time reaction control capabilityVSAvoidcomplexity of data acquisition and analysis system
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent implements self-service by enabling the reaction monitoring system to automatically identify transition points and determine reaction phase without requiring complex manual analysis or intervention. The system uses automated algorithms to process the raw photometric data, identify characteristic phase transitions, and provide real-time reaction status information, thereby achieving real-time control capability while keeping the operational complexity manageable through automation.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent segments the complex data analysis task into simpler sub-tasks: detecting baseline phase, identifying exponential growth phase, and recognizing plateau phase. By dividing the overall analysis into these manageable segments with distinct identification criteria, the system achieves real-time monitoring capability without requiring excessively complex analysis algorithms, thus balancing productivity improvement with acceptable system complexity.

Inventive Principle:
Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Accurately identifies transition points in chemical reactions, enhancing the precision of determining reaction product production and magnitude, and enables real-time control operations.

Implementation Method 1

In photometric methods such as QF-PCR, photometry is utilised for real-time detection and quantification of a reaction product.

Methodology Applied
Scientific EffectPhotometry: Absorption Spectroscopy

Implementation Method 2

targeted photoactive probes are utilised in the chemical reaction, to produce a photometric effect (i.e. light) detectable by an optical sensor

Methodology Applied
Scientific EffectFluorescence: Fluorescence

Data Source

PatentUS12467084B2Rate based identification of reaction points
Publication Date: 2025.11.11 AZURE VAULT LTD
  • US12467084B2 patent drawing
  • US12467084B2 patent drawing
  • US12467084B2 patent drawing

AI summary

An apparatus for identifying transition points in a chemical reaction, the apparatus comprising: a property value receiver, configured to receive a plurality of values of a physical property of the chemical reaction, a function calculator, associated with the property value receiver, configured to calculate a function and verify that the function has a line of best fit with a same slope as a linear function connecting two of the received values, the two values pertaining to a start and end of a time period, a difference calculator, associated with the function calculator, configured to calculate a difference between the calculated function and a plurality of the received values pertaining to the time period having the start and end, and a transition point identifier, associated with the difference calculator, configured to identify at least one transition point of the chemical reaction, using the calculated difference.